modified_roseospira_medium

CultureMech:007755 bacterial COMPLEX LIQUID
Original name: Modified Roseospira Medium

Overview

External ontology
TOGO:M1227 — Modified Roseospira Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1227 Original source: JCM - JCM_M1145 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1145

Composition

flowchart LR
    CultureMech_007755["modified_roseospira_medium"]
    ing0_Ferric_citrate__0_1___w_v_["Ferric citrate (0.1%, w/v)"]
    CultureMech_007755 --> ing0_Ferric_citrate__0_1___w_v_
    ing1_Distilled_water["Distilled water"]
    CultureMech_007755 --> ing1_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing1_Distilled_water -.-> CHEBI_15377
    ing2_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007755 --> ing2_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_7H2O -.-> CHEBI_31795
    ing3_Yeast_extract["Yeast extract"]
    CultureMech_007755 --> ing3_Yeast_extract
    ing4_NaCl["NaCl"]
    CultureMech_007755 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007755 --> ing5_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_2H2O -.-> CHEBI_86158
    ing6_KH2PO4["KH2PO4"]
    CultureMech_007755 --> ing6_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing6_KH2PO4 -.-> CHEBI_63036
    ing7_NH4Cl["NH4Cl"]
    CultureMech_007755 --> ing7_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing7_NH4Cl -.-> CHEBI_31206
    ing8_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_007755 --> ing8_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing8_Sodium_pyruvate -.-> CHEBI_50144
    ing9_NaHCO3["NaHCO3"]
    CultureMech_007755 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_Na2S_9H2O["Na2S・9H2O"]
    CultureMech_007755 --> ing10_Na2S_9H2O
    CHEBI_76209["CHEBI:76209"]
    ing10_Na2S_9H2O -.-> CHEBI_76209
    ing11_Na2S2O3["Na2S2O3"]
    CultureMech_007755 --> ing11_Na2S2O3
    CHEBI_32150["CHEBI:32150"]
    ing11_Na2S2O3 -.-> CHEBI_32150

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 Ferric citrate (0.1%, w/v) 5 G_PER_L Role: Mineral source; Properties: Defined component, Organic compound, Solution
2 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
3 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
1.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
4 Yeast extract 0.4 G_PER_L Role: Nutrient source; Properties: Undefined component
5 NaCl
Cl.Na 58.443 g/mol
20 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
6 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.15 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
7 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
8 NH4Cl
Cl.H4N 53.492 g/mol
0.6 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
9 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
3 G_PER_L CHEBI:50144
sodium pyruvate
 Role: Carbon source; Properties: Defined component, Simple component, Organic compound
10 NaHCO3
CHO3.Na 84.006 g/mol
variable VARIABLE CHEBI:32139
sodium hydrogencarbonate
 Role: Buffer; Properties: Defined component, Inorganic compound, Solution
11 Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
variable VARIABLE CHEBI:76209
sodium sulfide nonahydrate
 Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound
12 Na2S2O3
5H2O.2Na.O3S2 248.186 g/mol
variable VARIABLE CHEBI:32150
sodium thiosulfate pentahydrate
 Properties: Defined component, Inorganic compound, Simple component

Solutions

vitamin B12 solution

Dose: 1 G_PER_L

See mediadive_6105_Vitamin_B12_solution.yaml for composition

Micronutrient solution (SL7) (see Medium [M572])

Dose: 1 G_PER_L

Cross-reference to Medium M572

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:54.172246Z togo-import Imported from TOGO Medium Source: JCM, ID: M1227
2026-01-28T19:48:15.222585Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: NaHCO3; Added default concentration for ingredient: Na2S・9H2O; Added default concentration for ingredient: Na2S2O3
2026-03-09T08:04:25.365221+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:19:51.199120Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007755
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:09.619532+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Roseospira Medium" to "modified_roseospira_medium" for consistent matching
2026-05-16T09:03:08.632857+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:31.524683+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:22.044856+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:47.294723+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:06.914384+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 2dab67a37ceb8cea59e37798ee96bf2f2d30dace03256051d9e0552d94ff3eda