methanomassiliicoccus_alvus_medium

CultureMech:007760 archaea COMPLEX LIQUID
Original name: Methanomassiliicoccus Alvus Medium

Overview

External ontology
TOGO:M1231 — Methanomassiliicoccus Alvus Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1231 Original source: JCM - JCM_M1149 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1149

Composition

flowchart LR
    CultureMech_007760["methanomassiliicoccus_alvus_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007760 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007760 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_007760 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007760 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_KH2PO4["KH2PO4"]
    CultureMech_007760 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_NH4Cl["NH4Cl"]
    CultureMech_007760 --> ing5_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing5_NH4Cl -.-> CHEBI_31206
    ing6_Resazurin["Resazurin"]
    CultureMech_007760 --> ing6_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing6_Resazurin -.-> CHEBI_8806
    ing7_Sodium_acetate_3H2O["Sodium acetate・3H2O"]
    CultureMech_007760 --> ing7_Sodium_acetate_3H2O
    CHEBI_32954["CHEBI:32954"]
    ing7_Sodium_acetate_3H2O -.-> CHEBI_32954
    ing8_L_Cysteine_HCl_H2O["L-Cysteine・HCl・H2O"]
    CultureMech_007760 --> ing8_L_Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing8_L_Cysteine_HCl_H2O -.-> CHEBI_91248
    ing9_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_007760 --> ing9_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing9_Carbon_dioxide_gas -.-> CHEBI_16526
    ing10_Hydrogen_gas["Hydrogen gas"]
    CultureMech_007760 --> ing10_Hydrogen_gas
    CHEBI_18276["CHEBI:18276"]
    ing10_Hydrogen_gas -.-> CHEBI_18276

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
980 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.4 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
4 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.05 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
5 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
6 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
7 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Organic compound, Simple component, Defined component
8 Sodium acetate・3H2O
C2H3O2.Na 82.034 g/mol
2.7 G_PER_L CHEBI:32954
sodium acetate
 Role: Buffer; Properties: Simple component, Organic compound, Defined component
9 L-Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Defined component, Organic compound, Simple component
10 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
11 Hydrogen gas
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
 Properties: ガス

Solutions

Trace element solution (see Medium [M439])

Dose: 1 G_PER_L

Cross-reference to Medium M439

Selenite--tungstate solution (see Medium [M431])

Dose: 1 G_PER_L

Cross-reference to Medium M431

8% NaHCO3 solution*

Dose: 20 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

5% Na2S・9H2O solution

Dose: 8 G_PER_L

Role: Reducing agent; Properties: Defined component, Solution, Inorganic compound

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:54.183435Z togo-import Imported from TOGO Medium Source: JCM, ID: M1231
2026-01-28T19:48:40.544961Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas
2026-03-09T08:05:08.335120+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 4 solution(s) from ingredients
2026-03-13T06:19:51.253791Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007760
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:52.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:09.670352+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Methanomassiliicoccus Alvus Medium" to "methanomassiliicoccus_alvus_medium" for consistent matching
2026-05-16T09:03:08.689887+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:31.582558+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:22.104797+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:47.320060+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:24.797573+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint da793f7c7684a1574c9f2186e9b236b0f913f78ac26383619480e463b7b36305