k_medium

CultureMech:007765 bacterial COMPLEX SOLID_AGAR
Original name: K Medium

Overview

External ontology
TOGO:M1236 — K Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1236 Original source: JCM - JCM_M1154 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1154

Composition

flowchart LR
    CultureMech_007765["k_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007765 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007765 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_007765 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_KH2PO4["KH2PO4"]
    CultureMech_007765 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_FeSO4_7H2O["FeSO4・7H2O"]
    CultureMech_007765 --> ing4_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing4_FeSO4_7H2O -.-> CHEBI_75836
    ing5__NH4_2SO4["(NH4)2SO4"]
    CultureMech_007765 --> ing5__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing5__NH4_2SO4 -.-> CHEBI_62946
    ing6_Methanol["Methanol"]
    CultureMech_007765 --> ing6_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing6_Methanol -.-> CHEBI_17790
    ing7_Agar["Agar"]
    CultureMech_007765 --> ing7_Agar
    CHEBI_2509["CHEBI:2509"]
    ing7_Agar -.-> CHEBI_2509

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
990 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.125 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 NaCl
Cl.Na 58.443 g/mol
0.5 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
4 KH2PO4
H2O4P.K 136.084 g/mol
2 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
5 FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
0.02 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
6 (NH4)2SO4
2H4N.O4S 132.141 g/mol
2 G_PER_L CHEBI:62946
ammonium sulfate
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component
7 Methanol
CH4O 32.042 g/mol
10 G_PER_L CHEBI:17790
methanol
Role: Solvating media; Properties: Simple component, Organic compound, Defined component
8 Agar 15 G_PER_L CHEBI:2509
agar
Role: Solidifying component

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:54.191203Z togo-import Imported from TOGO Medium Source: JCM, ID: M1236
2026-01-28T19:48:30.773166Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:51.286320Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007765
2026-03-17T06:30:30.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:09.705234+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "K Medium" to "k_medium" for consistent matching
2026-06-05T07:40:31.606436+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:22.122524+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:47.329528+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:06.967509+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 50d69f81db10b34c6d5809941c4ff8befba28ca7317f08526b34d5b45ffe2ff3