Overview
- External ontology
- TOGO:M1240 — Ghiorsea Bivora Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1240 Original source: JCM - JCM_M1158 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1158
Composition
flowchart LR
CultureMech_007770["ghiorsea_bivora_medium"]
ing0_NaHCO3["NaHCO3"]
CultureMech_007770 --> ing0_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing0_NaHCO3 -.-> CHEBI_32139
ing1_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007770 --> ing1_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing1_Carbon_dioxide_gas -.-> CHEBI_16526
ing2_Nitrogen_gas["Nitrogen gas"]
CultureMech_007770 --> ing2_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing2_Nitrogen_gas -.-> CHEBI_17997
ing3_Modified_trace_vitamins__see_below_["Modified trace vitamins (see below)"]
CultureMech_007770 --> ing3_Modified_trace_vitamins__see_below_
ing4_H2["H2"]
CultureMech_007770 --> ing4_H2
CHEBI_18276["CHEBI:18276"]
ing4_H2 -.-> CHEBI_18276
ing5_air["air"]
CultureMech_007770 --> ing5_air
ing6_Distilled_water["Distilled water"]
CultureMech_007770 --> ing6_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing6_Distilled_water -.-> CHEBI_15377
ing7_KH2PO4["KH2PO4"]
CultureMech_007770 --> ing7_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing7_KH2PO4 -.-> CHEBI_63036
ing8_Biotin["Biotin"]
CultureMech_007770 --> ing8_Biotin
CHEBI_15956["CHEBI:15956"]
ing8_Biotin -.-> CHEBI_15956
ing9_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_007770 --> ing9_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing9_p_Aminobenzoic_acid -.-> CHEBI_30753
ing10_Thiamine_HCl["Thiamine・HCl"]
CultureMech_007770 --> ing10_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing10_Thiamine_HCl -.-> CHEBI_49105
ing11_Pyridoxine_HCl["Pyridoxine・HCl"]
CultureMech_007770 --> ing11_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing11_Pyridoxine_HCl -.-> CHEBI_30961
ing12_Folic_acid["Folic acid"]
CultureMech_007770 --> ing12_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing12_Folic_acid -.-> CHEBI_27470
ing13_Vitamin_B12["Vitamin B12"]
CultureMech_007770 --> ing13_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing13_Vitamin_B12 -.-> CHEBI_176843
ing14_Riboflavin["Riboflavin"]
CultureMech_007770 --> ing14_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing14_Riboflavin -.-> CHEBI_17015
ing15_Nicotinic_acid["Nicotinic acid"]
CultureMech_007770 --> ing15_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing15_Nicotinic_acid -.-> CHEBI_15940
ing16_Lipoic_acid["Lipoic acid"]
CultureMech_007770 --> ing16_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing16_Lipoic_acid -.-> CHEBI_16494
ing17_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
CultureMech_007770 --> ing17_DL_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing17_DL_Calcium_pantothenate -.-> CHEBI_31345
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.68 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 3 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 4 | Modified trace vitamins (see below) | 1 G_PER_L | ||
| 5 |
H2
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 6 | air | variable VARIABLE | Properties: ガス | |
| 7 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 8 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.9 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 9 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Defined component, Simple component, Organic compound |
| 10 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Simple component, Defined component |
| 11 |
Thiamine・HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
5 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Defined component, Organic compound, Simple component |
| 12 |
Pyridoxine・HCl
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Defined component, Simple component, Organic compound |
| 13 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Simple component, Defined component |
| 14 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Simple component, Organic compound, Defined component |
| 15 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Defined component, Organic compound, Simple component |
| 16 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Defined component, Simple component, Organic compound |
| 17 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Defined component, Simple component, Organic compound |
| 18 |
DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
5 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
Role: Vitamin; Properties: Defined component, Organic compound, Simple component |
Solutions
Artificial saltwater (see Medium [M642])
Dose: 1 G_PER_L
Cross-reference to Medium M642
Wolfe's mineral solution (see Medium [M257])
Dose: 1 G_PER_L
Cross-reference to Medium M257
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1240_Ghiorsea_Bivora_Medium (CultureMech:007770)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.201523Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1240 |
| 2026-01-28T19:48:36.898437Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: H2; Added default concentration for ingredient: air |
| 2026-03-09T08:05:01.593913+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:19:51.326270Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007770 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:09.748214+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Ghiorsea Bivora Medium" to "ghiorsea_bivora_medium" for consistent matching |
| 2026-05-16T09:03:08.753030+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:31.655267+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:30.119096+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 1 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:47.346545+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:07.017173+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint ec9e98cb8f5c1d962e054c7facb207c961955dce2e007cc22dfc9190b0ae778f |