m_medium

CultureMech:007795 bacterial COMPLEX LIQUID
Original name: M Medium

Overview

External ontology
TOGO:M1263 — M Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1263 Original source: JCM - JCM_M1179 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1179

Composition

flowchart LR
    CultureMech_007795["m_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007795 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007795 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_007795 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_KH2PO4["KH2PO4"]
    CultureMech_007795 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_KNO3["KNO3"]
    CultureMech_007795 --> ing4_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing4_KNO3 -.-> CHEBI_63043
    ing5_Methanol["Methanol"]
    CultureMech_007795 --> ing5_Methanol
    CHEBI_17790["CHEBI:17790"]
    ing5_Methanol -.-> CHEBI_17790

Ingredients (6)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
935 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.22 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 NaCl
Cl.Na 58.443 g/mol
30 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
4 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
5 KNO3
K.NO3 101.102 g/mol
1 G_PER_L CHEBI:63043
potassium nitrate
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
6 Methanol
CH4O 32.042 g/mol
5 G_PER_L CHEBI:17790
methanol
Role: Solvating media; Properties: Simple component, Organic compound, Defined component

Solutions

2 M NaHCO3 solution

Dose: 50 G_PER_L

Role: Buffer; Properties: Defined component, Inorganic compound, Solution

1 M Na2CO3 solution

Dose: 10 G_PER_L

Role: Buffer; Properties: Defined component, Solution, Inorganic compound

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:54.246940Z togo-import Imported from TOGO Medium Source: JCM, ID: M1263
2026-01-28T19:48:21.947838Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:36.640632+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T06:19:51.531026Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007795
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:30:12.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:09.957675+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "M Medium" to "m_medium" for consistent matching
2026-05-16T09:03:08.956174+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:31.873469+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:22.337812+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:47.435859+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:07.256300+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 965de86fff2d7409fd79b28ed63d9dd27cbed9712a55bedbc2e89dd3ff47a9ff