Overview
- External ontology
- TOGO:M1277 — Geothermobacter Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1277 Original source: JCM - JCM_M1192 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1192
Composition
flowchart LR
CultureMech_007810["geothermobacter_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007810 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_007810 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007810 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_007810 --> ing3_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_6H2O -.-> CHEBI_86345
ing4_KCl["KCl"]
CultureMech_007810 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_007810 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007810 --> ing6_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing6_Carbon_dioxide_gas -.-> CHEBI_16526
ing7_Hydrogen_gas["Hydrogen gas"]
CultureMech_007810 --> ing7_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing7_Hydrogen_gas -.-> CHEBI_18276
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
840 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
15 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.33 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound |
| 7 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 8 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
Solutions
FeCl2 solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
Trace element solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
Selenite--tungstate solution (see Medium [M431])
Dose: 1 G_PER_L
Cross-reference to Medium M431
8% NaHCO3 solution*
Dose: 30 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
0.1 M KH2PO4 solution
Dose: 20 G_PER_L
Role: Buffer; Properties: Defined component, Solution, Inorganic compound
Iron(III) citrate solution (see Medium [M1072])
Dose: 100 G_PER_L
Cross-reference to Medium M1072
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1277_Geothermobacter_Medium (CultureMech:007810)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.273689Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1277 |
| 2026-01-28T19:48:32.550701Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:04:53.996851+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 7 solution(s) from ingredients |
| 2026-03-13T06:19:51.643671Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007810 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:34.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.076603+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Geothermobacter Medium" to "geothermobacter_medium" for consistent matching |
| 2026-05-16T09:03:09.045050+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:31.966730+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.461700+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:07.479615+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 23e4cbc2a42ebb408ed98c6aa6e7c40da35263183ca968850820c50c60145587 |