Overview
- External ontology
- TOGO:M1286 — ELBE Methanotroph Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1286 Original source: JCM - JCM_M1200-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1200
Composition
flowchart LR
CultureMech_007820["elbe_methanotroph_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007820 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007820 --> ing1_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_2H2O -.-> CHEBI_86158
ing2_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_007820 --> ing2_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_6H2O -.-> CHEBI_86345
ing3_KCl["KCl"]
CultureMech_007820 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NaNO3["NaNO3"]
CultureMech_007820 --> ing4_NaNO3
CHEBI_63005["CHEBI:63005"]
ing4_NaNO3 -.-> CHEBI_63005
ing5_2_M_HEPES_buffer__pH_7_0_["2 M HEPES buffer (pH 7.0)"]
CultureMech_007820 --> ing5_2_M_HEPES_buffer__pH_7_0_
ing6_methane["methane"]
CultureMech_007820 --> ing6_methane
ing7_agar["agar"]
CultureMech_007820 --> ing7_agar
CHEBI_2509["CHEBI:2509"]
ing7_agar -.-> CHEBI_2509
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
992 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.12 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.93 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
KCl
Cl.K 74.551 g/mol
|
1 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
NaNO3
NO3.Na 84.994 g/mol
|
0.17 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component |
| 6 | 2 M HEPES buffer (pH 7.0) | 5 G_PER_L | Role: Buffer; Properties: Defined component, Solution | |
| 7 | methane | variable VARIABLE | Properties: Gas | |
| 8 | agar | variable VARIABLE |
CHEBI:2509
agar
|
Role: Solidifying component |
Solutions
Phosphate buffer (see Medium [M560])
Dose: 0.15 G_PER_L
Cross-reference to Medium M560
FeCl2 solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
Trace element solution (see Medium [M180])
Dose: 1 G_PER_L
Cross-reference to Medium M180
Vitamin solution (see Medium [M560])
Dose: 1 G_PER_L
Cross-reference to Medium M560
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1286_ELBE_Methanotroph_Medium (CultureMech:007820)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.289039Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1286 |
| 2026-01-28T19:48:40.801812Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: methane; Added default concentration for ingredient: agar |
| 2026-03-09T08:05:08.690080+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 4 solution(s) from ingredients |
| 2026-03-13T06:19:51.722464Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007820 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:52.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.156314+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ELBE Methanotroph Medium" to "elbe_methanotroph_medium" for consistent matching |
| 2026-05-16T09:03:09.132315+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:32.067287+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:07.571916+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0f131258acee0a0f28ad8ed454e3a7d830407739477d38dd6e921f5efca8f445 |