pyg_medium_j_modified

CultureMech:007835 bacterial COMPLEX LIQUID
Original name: PYG Medium (J), Modified

Overview

External ontology
TOGO:M1300 — PYG Medium (J), Modified
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1300 Original source: JCM - JCM_M1213 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1213

Composition

flowchart LR
    CultureMech_007835["pyg_medium_j_modified"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007835 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_007835 --> ing1_Yeast_extract
    ing2_KH2PO4["KH2PO4"]
    CultureMech_007835 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_Resazurin["Resazurin"]
    CultureMech_007835 --> ing3_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing3_Resazurin -.-> CHEBI_8806
    ing4_Tween_80["Tween 80"]
    CultureMech_007835 --> ing4_Tween_80
    CHEBI_53426["CHEBI:53426"]
    ing4_Tween_80 -.-> CHEBI_53426
    ing5_Glucose["Glucose"]
    CultureMech_007835 --> ing5_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing5_Glucose -.-> CHEBI_17234
    ing6_Beef_extract["Beef extract"]
    CultureMech_007835 --> ing6_Beef_extract
    ing7_Trypticase_peptone["Trypticase peptone"]
    CultureMech_007835 --> ing7_Trypticase_peptone
    ing8_Peptone["Peptone"]
    CultureMech_007835 --> ing8_Peptone
    ing9_CO2["CO2"]
    CultureMech_007835 --> ing9_CO2
    CHEBI_16526["CHEBI:16526"]
    ing9_CO2 -.-> CHEBI_16526
    ing10_N2["N2"]
    CultureMech_007835 --> ing10_N2
    CHEBI_17997["CHEBI:17997"]
    ing10_N2 -.-> CHEBI_17997
    ing11_ethanol["ethanol"]
    CultureMech_007835 --> ing11_ethanol
    CHEBI_16236["CHEBI:16236"]
    ing11_ethanol -.-> CHEBI_16236
    ing12_vitamin_K1["vitamin K1"]
    CultureMech_007835 --> ing12_vitamin_K1
    CHEBI_18067["CHEBI:18067"]
    ing12_vitamin_K1 -.-> CHEBI_18067

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
890 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound
2 Yeast extract 10 G_PER_L Role: Nutrient source; Properties: Undefined component
3 KH2PO4
H2O4P.K 136.084 g/mol
2 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
4 Resazurin
C12H7NO4 229.191 g/mol
1 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component
5 Tween 80
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H44O6 604.822 g/mol
1 G_PER_L CHEBI:53426
polysorbate 80
 Role: Surfactant; Properties: Organic compound, Simple component, Defined component
6 Glucose
C6H12O6 180.156 g/mol
5 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Organic compound, Simple component, Defined component
7 Beef extract 5 G_PER_L Properties: Undefined component
8 Trypticase peptone 5 G_PER_L Properties: Undefined component
9 Peptone 5 G_PER_L
10 CO2
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
11 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
12 ethanol
C2H6O 46.069 g/mol
20 G_PER_L CHEBI:16236
ethanol
 Role: Solvating media; Properties: Defined component, Organic compound, Simple component
13 vitamin K1
C31H46O2 450.707 g/mol
0.1 G_PER_L CHEBI:18067
phylloquinone
 Role: Vitamin; Properties: Solution, Organic compound, Defined component

Solutions

Salt solution (see Medium [M695])

Dose: 40 G_PER_L

Cross-reference to Medium M695

5% L-Cysteine・HCl・H2O solution

Dose: 10 G_PER_L

Properties: Defined component, Organic compound, Solution

Rumen fluid, clarified (see Medium [M258])

Dose: 50 G_PER_L

Cross-reference to Medium M258

Hemin solution (see Medium [M470])

Dose: 10 G_PER_L

Cross-reference to Medium M470

Vitamin K1 solution (see below)

Dose: 0.2 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:54.316871Z togo-import Imported from TOGO Medium Source: JCM, ID: M1300
2026-01-28T19:48:24.866180Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: CO2; Added default concentration for ingredient: N2
2026-03-09T08:04:41.108445+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 5 solution(s) from ingredients
2026-03-13T06:19:51.843446Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007835
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:18.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:10.280222+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PYG Medium (J), Modified" to "pyg_medium_j_modified" for consistent matching
2026-05-16T09:03:09.251852+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:32.188583+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:47.536152+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:07.698041+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint fc168187b2a8452893a871266b8cb0f5ef40a2186458f11837920fa537ee26d1