modified_haloalkaliphile_medium

CultureMech:007837 bacterial COMPLEX LIQUID
Original name: Modified Haloalkaliphile Medium

Overview

External ontology
TOGO:M1302 — Modified Haloalkaliphile Medium
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'haloalkaliphile_medium', 'source': 'TOGO', 'source_id': 'TOGO:M301', 'original_category': 'bacterial'}
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M1302 Original source: JCM - JCM_M1215 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1215

Composition

flowchart LR
    CultureMech_007837["modified_haloalkaliphile_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007837 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007837 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_007837 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_KCl["KCl"]
    CultureMech_007837 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_CaSO4_2H2O["CaSO4・2H2O"]
    CultureMech_007837 --> ing4_CaSO4_2H2O
    CHEBI_32583["CHEBI:32583"]
    ing4_CaSO4_2H2O -.-> CHEBI_32583
    ing5_Sodium_glutamate["Sodium glutamate"]
    CultureMech_007837 --> ing5_Sodium_glutamate
    CHEBI_64220["CHEBI:64220"]
    ing5_Sodium_glutamate -.-> CHEBI_64220
    ing6_Trisodium_citrate_2H2O["Trisodium citrate・2H2O"]
    CultureMech_007837 --> ing6_Trisodium_citrate_2H2O
    CHEBI_53258["CHEBI:53258"]
    ing6_Trisodium_citrate_2H2O -.-> CHEBI_53258
    ing7_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_007837 --> ing7_Yeast_extract__BD_Difco_
    ing8_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
    CultureMech_007837 --> ing8_Casamino_acids__BD_Difco_
    ing9_Na2CO3["Na2CO3"]
    CultureMech_007837 --> ing9_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing9_Na2CO3 -.-> CHEBI_29377

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1000.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 500.0, 500.0]
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.1 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
3 NaCl
Cl.Na 58.443 g/mol
200.0 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound [Merged 2 duplicates: 100.0, 100.0]
4 KCl
Cl.K 74.551 g/mol
1 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
5 CaSO4・2H2O
Ca.2H2O.O4S 172.171 g/mol
0.1 G_PER_L CHEBI:32583
calcium sulfate dihydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
6 Sodium glutamate
C5H8NO4.Na 169.112 g/mol
1 G_PER_L CHEBI:64220
monosodium glutamate
 Role: Nitrogen source; Properties: Organic compound, Simple component, Defined component
7 Trisodium citrate・2H2O
C6H5O7.3Na 258.069 g/mol
3 G_PER_L CHEBI:53258
sodium citrate
 Role: Buffer; Properties: Simple component, Organic compound, Defined component
8 Yeast extract (BD-Difco) 2 G_PER_L Properties: Undefined component
9 Casamino acids (BD-Difco) 2 G_PER_L Properties: Undefined component
10 Na2CO3
CO3.2Na 105.988 g/mol
80 G_PER_L CHEBI:29377
sodium carbonate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound

Solutions

Solution A

Dose: 500 G_PER_L

See mediadive_5342_Solution_A.yaml for composition

Solution B

Dose: 500 G_PER_L

See mediadive_5343_Solution_B.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-27T06:35:54.321374Z togo-import Imported from TOGO Medium Source: JCM, ID: M1302
2026-01-28T19:48:08.064061Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:12.973668+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T03:08:39.909770Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:51.862881Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007837
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:29:41.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:10.300637+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Haloalkaliphile Medium" to "modified_haloalkaliphile_medium" for consistent matching
2026-05-16T09:03:09.261514+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:32.198442+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:22.592557+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:47.540499+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:07.714769+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: TOGO_M1302_Modified_Haloalkaliphile_Medium, TOGO_M301_Haloalkaliphile_Medium
2026-08-06T03:05:07.714812+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 0a16d1c35e9b3a6b450cb5f4b0327f719527af61800ea5561e90a54d7e5a899b