Overview
- External ontology
- TOGO:M1303 — Modified Haloalkaliphile Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1303 Original source: JCM - JCM_M1215-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1215
Composition
flowchart LR
CultureMech_007838["modified_haloalkaliphile_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007838 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007838 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_007838 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_KCl["KCl"]
CultureMech_007838 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_CaSO4_2H2O["CaSO4・2H2O"]
CultureMech_007838 --> ing4_CaSO4_2H2O
CHEBI_32583["CHEBI:32583"]
ing4_CaSO4_2H2O -.-> CHEBI_32583
ing5_Sodium_glutamate["Sodium glutamate"]
CultureMech_007838 --> ing5_Sodium_glutamate
CHEBI_64220["CHEBI:64220"]
ing5_Sodium_glutamate -.-> CHEBI_64220
ing6_Trisodium_citrate_2H2O["Trisodium citrate・2H2O"]
CultureMech_007838 --> ing6_Trisodium_citrate_2H2O
CHEBI_53258["CHEBI:53258"]
ing6_Trisodium_citrate_2H2O -.-> CHEBI_53258
ing7_agar["agar"]
CultureMech_007838 --> ing7_agar
CHEBI_2509["CHEBI:2509"]
ing7_agar -.-> CHEBI_2509
ing8_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_007838 --> ing8_Yeast_extract__BD_Difco_
ing9_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_007838 --> ing9_Casamino_acids__BD_Difco_
ing10_Na2CO3["Na2CO3"]
CultureMech_007838 --> ing10_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing10_Na2CO3 -.-> CHEBI_29377
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 500.0, 500.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
200.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component [Merged 2 duplicates: 100.0, 100.0] |
| 4 |
KCl
Cl.K 74.551 g/mol
|
1 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
CaSO4・2H2O
Ca.2H2O.O4S 172.171 g/mol
|
0.1 G_PER_L |
CHEBI:32583
calcium sulfate dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
Sodium glutamate
C5H8NO4.Na 169.112 g/mol
|
1 G_PER_L |
CHEBI:64220
monosodium glutamate
|
Role: Nitrogen source; Properties: Organic compound, Simple component, Defined component |
| 7 |
Trisodium citrate・2H2O
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Simple component, Organic compound, Defined component |
| 8 | agar | 18 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 9 | Yeast extract (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 10 | Casamino acids (BD-Difco) | 2 G_PER_L | Properties: Undefined component | |
| 11 |
Na2CO3
CO3.2Na 105.988 g/mol
|
80 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
Solutions
Solution A
Dose: 500 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 500 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1303_Modified_Haloalkaliphile_Medium (CultureMech:007838)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.323197Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1303 |
| 2026-01-28T19:48:11.204116Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:18.355408+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:08:46.057755Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:51.870621Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007838 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.308787+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified Haloalkaliphile Medium" to "modified_haloalkaliphile_medium" for consistent matching |
| 2026-05-16T09:03:09.270832+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:32.207822+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:22.602587+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:47.544750+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:07.726301+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint fd00c96a7136d700f46be0a3c7fa6a603176e3eb0178cf1a5539bad1faa80ca7 |