modified_methanohalophilus_medium

CultureMech:007842 archaea COMPLEX LIQUID
Original name: Modified Methanohalophilus Medium

Overview

External ontology
TOGO:M1307 — Modified Methanohalophilus Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1307 Original source: JCM - JCM_M1218 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1218

Composition

flowchart LR
    CultureMech_007842["modified_methanohalophilus_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007842 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_007842 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007842 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_NH4Cl["NH4Cl"]
    CultureMech_007842 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_K2HPO4["K2HPO4"]
    CultureMech_007842 --> ing4_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing4_K2HPO4 -.-> CHEBI_131527
    ing5_Resazurin["Resazurin"]
    CultureMech_007842 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_007842 --> ing6_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_6H2O -.-> CHEBI_86345
    ing7_KCl["KCl"]
    CultureMech_007842 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
    CultureMech_007842 --> ing8_Trypticase_peptone__BD_BBL_
    ing9_Yeast_extract__Oxoid_["Yeast extract (Oxoid)"]
    CultureMech_007842 --> ing9_Yeast_extract__Oxoid_
    ing10_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_007842 --> ing10_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing10_Carbon_dioxide_gas -.-> CHEBI_16526
    ing11_Nitrogen_gas["Nitrogen gas"]
    CultureMech_007842 --> ing11_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing11_Nitrogen_gas -.-> CHEBI_17997
    ing12_N2["N2"]
    CultureMech_007842 --> ing12_N2
    ing12_N2 -.-> CHEBI_17997

Ingredients (13)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
920 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 NaCl
Cl.Na 58.443 g/mol
120 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.4 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
4 NH4Cl
Cl.H4N 53.492 g/mol
1 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component
5 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Simple component, Inorganic compound, Defined component
6 Resazurin
C12H7NO4 229.191 g/mol
0.5 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Defined component, Simple component, Organic compound
7 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
6 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
8 KCl
Cl.K 74.551 g/mol
1.5 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
9 Trypticase peptone (BD-BBL) 2 G_PER_L Properties: Undefined component
10 Yeast extract (Oxoid) 2 G_PER_L Properties: Undefined component, Complex component
11 Carbon dioxide gas
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: ガス
12 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス
13 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: ガス

Solutions

Trace minerals (see Medium [M142])

Dose: 10 G_PER_L

Cross-reference to Medium M142

Trace vitamins (see Medium [M190])

Dose: 10 G_PER_L

Cross-reference to Medium M190

8% NaHCO3 solution

Dose: 50 G_PER_L

Role: Buffer; Properties: Inorganic compound, Solution, Defined component

1.4% Coenzyme M solution

Dose: 10 G_PER_L

Role: Growth factor; Properties: Defined component, Organic compound, Solution

1 M Trimethylamine・HCl solution

Dose: 20 G_PER_L

Role: Nutrient source; Properties: Complex component, Solution, Defined component

5% Na2S・9H2O solution

Dose: 5 G_PER_L

Role: Reducing agent; Properties: Defined component, Inorganic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:54.328892Z togo-import Imported from TOGO Medium Source: JCM, ID: M1307
2026-01-28T19:48:32.440865Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: N2
2026-03-09T08:04:53.844322+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 6 solution(s) from ingredients
2026-03-13T06:19:51.894836Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007842
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:34.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:10.333585+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Methanohalophilus Medium" to "modified_methanohalophilus_medium" for consistent matching
2026-05-16T09:04:25.871872+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:41:37.277113+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.787667+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:24.879882+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 9bb0fbd9be88eca9d629f1baeaaf4937ac1dcf36b9048af4637ef1b39e9d109d