Overview
- External ontology
- TOGO:M1309 — Methylotroph Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1309 Original source: JCM - JCM_M1219-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1219
Composition
flowchart LR
CultureMech_007844["methylotroph_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007844 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007844 --> ing1_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing1_CaCl2_2H2O -.-> CHEBI_86158
ing2_KH2PO4["KH2PO4"]
CultureMech_007844 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_007844 --> ing3_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_6H2O -.-> CHEBI_86345
ing4_NaNO3["NaNO3"]
CultureMech_007844 --> ing4_NaNO3
CHEBI_63005["CHEBI:63005"]
ing4_NaNO3 -.-> CHEBI_63005
ing5_Na2SO4["Na2SO4"]
CultureMech_007844 --> ing5_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing5_Na2SO4 -.-> CHEBI_32149
ing6_HEPES["HEPES"]
CultureMech_007844 --> ing6_HEPES
CHEBI_46756["CHEBI:46756"]
ing6_HEPES -.-> CHEBI_46756
ing7_agar["agar"]
CultureMech_007844 --> ing7_agar
CHEBI_2509["CHEBI:2509"]
ing7_agar -.-> CHEBI_2509
ing8_KOH["KOH"]
CultureMech_007844 --> ing8_KOH
CHEBI_32035["CHEBI:32035"]
ing8_KOH -.-> CHEBI_32035
ing9_Methanol["Methanol"]
CultureMech_007844 --> ing9_Methanol
CHEBI_17790["CHEBI:17790"]
ing9_Methanol -.-> CHEBI_17790
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
970 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.07 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.27 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 5 |
NaNO3
NO3.Na 84.994 g/mol
|
0.42 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component |
| 6 |
Na2SO4
2Na.O4S 142.043 g/mol
|
0.014 G_PER_L |
CHEBI:32149
sodium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 7 | HEPES | 4.77 G_PER_L |
CHEBI:46756
HEPES
|
Role: Buffer; Properties: Simple component, Organic compound, Defined component |
| 8 | agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 9 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
| 10 |
Methanol
CH4O 32.042 g/mol
|
0.81 G_PER_L |
CHEBI:17790
methanol
|
Role: Solvating media; Properties: Organic compound, Simple component, Defined component |
Solutions
Trace element solution (see Medium [M278])
Dose: 10 G_PER_L
Cross-reference to Medium M278
Se/W solution (see Medium [M278])
Dose: 10 G_PER_L
Cross-reference to Medium M278
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
10 mM CuCl2 solution
Dose: 2 G_PER_L
Role: Mineral source
10 mM CeCl3 solution
Dose: 2 G_PER_L
Role: Mineral source
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1309_Methylotroph_Medium (CultureMech:007844)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.332732Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1309 |
| 2026-01-28T19:48:21.580294Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: KOH |
| 2026-03-09T08:04:36.093081+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T06:19:51.912150Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007844 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:11.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.352000+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Methylotroph Medium" to "methylotroph_medium" for consistent matching |
| 2026-05-16T09:03:09.308896+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:32.258153+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.557059+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:07.860623+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint df2788aec6830484421793ba7eb5bd792a01adecfe1e9cd5399f80051c53fec5 |