Overview
- External ontology
- TOGO:M1322 — NSY Medium For Polynucleobacter
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1322 Original source: JCM - JCM_M1230 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1230
Composition
flowchart LR
CultureMech_007859["nsy_medium_for_polynucleobacter"]
ing0_Distilled_water["Distilled water"]
CultureMech_007859 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007859 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_K2HPO4["K2HPO4"]
CultureMech_007859 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_KCl["KCl"]
CultureMech_007859 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NaHCO3["NaHCO3"]
CultureMech_007859 --> ing4_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing4_NaHCO3 -.-> CHEBI_32139
ing5_Ca_NO3_2_4H2O["Ca(NO3)2・4H2O"]
CultureMech_007859 --> ing5_Ca_NO3_2_4H2O
CHEBI_86159["CHEBI:86159"]
ing5_Ca_NO3_2_4H2O -.-> CHEBI_86159
ing6_Agar["Agar"]
CultureMech_007859 --> ing6_Agar
CHEBI_2509["CHEBI:2509"]
ing6_Agar -.-> CHEBI_2509
ing7_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_007859 --> ing7_Yeast_extract__BD_Difco_
ing8_Nutrient_broth["Nutrient broth"]
CultureMech_007859 --> ing8_Nutrient_broth
ing9_Phytone_peptone__BD_BBL_["Phytone peptone (BD-BBL)"]
CultureMech_007859 --> ing9_Phytone_peptone__BD_BBL_
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
75 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
3.7 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
KCl
Cl.K 74.551 g/mol
|
5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
NaHCO3
CHO3.Na 84.006 g/mol
|
16 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Simple component, Defined component, Inorganic compound |
| 6 |
Ca(NO3)2・4H2O
Ca.4H2O.2NO3 236.146 g/mol
|
43 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 7 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 8 | Yeast extract (BD-Difco) | 1 G_PER_L | Properties: Undefined component | |
| 9 | Nutrient broth | 1 G_PER_L | Properties: Undefined component, Complex component | |
| 10 | Phytone peptone (BD-BBL) | 1 G_PER_L | Properties: Undefined component |
Solutions
SL--4 trace element solution (see Medium [M335])
Dose: 0.1 G_PER_L
Cross-reference to Medium M335
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1322_NSY_Medium_For_Polynucleobacter (CultureMech:007859)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.357827Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1322 |
| 2026-01-28T19:48:33.121300Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:54.853806+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:52.020965Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007859 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:35.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.465451+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NSY Medium For Polynucleobacter" to "nsy_medium_for_polynucleobacter" for consistent matching |
| 2026-05-16T09:03:09.393238+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:32.351987+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:22.707699+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:47.588536+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:08.010305+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 38a269c33f87569463f5747c7e4dbcec713cee8875acf8dadb5bb004fa027ea5 |