Overview
- External ontology
- TOGO:M1341 — R2A Medium With 25% Salt
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1341 Original source: JCM - JCM_M1247 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1247
Composition
flowchart LR
CultureMech_007877["r2a_medium_with_25_salt"]
ing0_Distilled_water["Distilled water"]
CultureMech_007877 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007877 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_K2HPO4["K2HPO4"]
CultureMech_007877 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_007877 --> ing3_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing3_Sodium_pyruvate -.-> CHEBI_50144
ing4_Glucose["Glucose"]
CultureMech_007877 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Soluble_starch["Soluble starch"]
CultureMech_007877 --> ing5_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing5_Soluble_starch -.-> CHEBI_28017
ing6_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_007877 --> ing6_Yeast_extract__BD_Difco_
ing7_Proteose_peptone_No__3__BD_Difco_["Proteose peptone No. 3 (BD-Difco)"]
CultureMech_007877 --> ing7_Proteose_peptone_No__3__BD_Difco_
ing8_Casamino_acids__BD_Difco_["Casamino acids (BD-Difco)"]
CultureMech_007877 --> ing8_Casamino_acids__BD_Difco_
ing9_NaCl["NaCl"]
CultureMech_007877 --> ing9_NaCl
CHEBI_26710["CHEBI:26710"]
ing9_NaCl -.-> CHEBI_26710
ing10_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007877 --> ing10_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing10_CaCl2_2H2O -.-> CHEBI_86158
ing11_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_007877 --> ing11_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing11_MgCl2_6H2O -.-> CHEBI_86345
ing12_KCl["KCl"]
CultureMech_007877 --> ing12_KCl
CHEBI_32588["CHEBI:32588"]
ing12_KCl -.-> CHEBI_32588
ing13_NaHCO3["NaHCO3"]
CultureMech_007877 --> ing13_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing13_NaHCO3 -.-> CHEBI_32139
ing14_NaBr["NaBr"]
CultureMech_007877 --> ing14_NaBr
CHEBI_63004["CHEBI:63004"]
ing14_NaBr -.-> CHEBI_63004
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
168.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 167.0, 1.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
61.05 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.05, 61.0] |
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.3 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 6 | Soluble starch | 0.5 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 7 | Yeast extract (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 8 | Proteose peptone No. 3 (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 9 | Casamino acids (BD-Difco) | 0.5 G_PER_L | Properties: Undefined component | |
| 10 |
NaCl
Cl.Na 58.443 g/mol
|
234 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1.3 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
39 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
KCl
Cl.K 74.551 g/mol
|
6 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.2 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
NaBr
Br.Na 102.894 g/mol
|
0.7 G_PER_L |
CHEBI:63004
sodium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
30% Seawater solution (see below)
Dose: 833 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1341_R2A_Medium_With_25_Salt (CultureMech:007877)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.385525Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1341 |
| 2026-01-28T19:48:24.305289Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:40.173111+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:10.112037Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:52.139728Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007877 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:16.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.588522+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "R2A Medium With 25% Salt" to "r2a_medium_with_25_salt" for consistent matching |
| 2026-05-16T09:03:09.501833+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:32.471462+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:22.807888+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:47.621961+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:08.143826+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9b9d630d39771104109663227705f13bdb813d8a73d4592fdbe63a4aab985182 |