Overview
- External ontology
- TOGO:M1350 — Haloarchaeal Complex Medium PH 9.0
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1350 Original source: JCM - JCM_M1254-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1254
Composition
flowchart LR
CultureMech_007887["haloarchaeal_complex_medium_ph_9_0"]
ing0_Distilled_water["Distilled water"]
CultureMech_007887 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_007887 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_007887 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_K2HPO4["K2HPO4"]
CultureMech_007887 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_007887 --> ing4_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_6H2O -.-> CHEBI_86345
ing5_KCl["KCl"]
CultureMech_007887 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_Sodium_acetate["Sodium acetate"]
CultureMech_007887 --> ing6_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing6_Sodium_acetate -.-> CHEBI_32954
ing7_NaNO3["NaNO3"]
CultureMech_007887 --> ing7_NaNO3
CHEBI_63005["CHEBI:63005"]
ing7_NaNO3 -.-> CHEBI_63005
ing8_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_007887 --> ing8_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing8_Sodium_pyruvate -.-> CHEBI_50144
ing9_Sodium_succinate["Sodium succinate"]
CultureMech_007887 --> ing9_Sodium_succinate
CHEBI_63675["CHEBI:63675"]
ing9_Sodium_succinate -.-> CHEBI_63675
ing10_Sodium_lactate["Sodium lactate"]
CultureMech_007887 --> ing10_Sodium_lactate
CHEBI_75228["CHEBI:75228"]
ing10_Sodium_lactate -.-> CHEBI_75228
ing11_Sodium_citrate["Sodium citrate"]
CultureMech_007887 --> ing11_Sodium_citrate
CHEBI_53258["CHEBI:53258"]
ing11_Sodium_citrate -.-> CHEBI_53258
ing12_CaCl2_H2O["CaCl2・H2O"]
CultureMech_007887 --> ing12_CaCl2_H2O
CHEBI_3312["CHEBI:3312"]
ing12_CaCl2_H2O -.-> CHEBI_3312
ing13_Fish_peptone__Fluka_["Fish peptone (Fluka)"]
CultureMech_007887 --> ing13_Fish_peptone__Fluka_
ing14_agar["agar"]
CultureMech_007887 --> ing14_agar
CHEBI_2509["CHEBI:2509"]
ing14_agar -.-> CHEBI_2509
ing15_Sodium_formate["Sodium formate"]
CultureMech_007887 --> ing15_Sodium_formate
CHEBI_62965["CHEBI:62965"]
ing15_Sodium_formate -.-> CHEBI_62965
ing16_Sodium_malate["Sodium malate"]
CultureMech_007887 --> ing16_Sodium_malate
CHEBI_91260["CHEBI:91260"]
ing16_Sodium_malate -.-> CHEBI_91260
ing17_Sodium_fumarate["Sodium fumarate"]
CultureMech_007887 --> ing17_Sodium_fumarate
CHEBI_115156["CHEBI:115156"]
ing17_Sodium_fumarate -.-> CHEBI_115156
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Yeast extract | 0.05 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
184 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.2 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 5 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
KCl
Cl.K 74.551 g/mol
|
5.4 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.2 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component |
| 8 |
NaNO3
NO3.Na 84.994 g/mol
|
0.5 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 9 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.2 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Organic compound, Defined component, Simple component |
| 10 |
Sodium succinate
C4H4O4.2Na 162.052 g/mol
|
0.2 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
Role: Growth factor; Properties: Defined component, Organic compound, Simple component |
| 11 |
Sodium lactate
C3H5O3.Na 112.06 g/mol
|
0.2 G_PER_L |
CHEBI:75228
sodium lactate
|
Role: Carbon source; Properties: Simple component, Defined component, Organic compound |
| 12 |
Sodium citrate
C6H5O7.3Na 258.069 g/mol
|
0.2 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Simple component, Organic compound, Defined component |
| 13 |
CaCl2・H2O
Ca.2Cl 110.984 g/mol
|
0.1 G_PER_L |
CHEBI:3312
calcium dichloride
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 14 | Fish peptone (Fluka) | 0.25 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 15 | agar | 20 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 16 |
Sodium formate
CHO2.Na 68.007 g/mol
|
0.2 G_PER_L |
CHEBI:62965
sodium formate
|
Properties: Defined component, Inorganic compound, Simple component |
| 17 | Sodium malate | 0.2 G_PER_L |
CHEBI:91260
disodium malate
|
Properties: Defined component, Organic compound, Simple component |
| 18 |
Sodium fumarate
C4H2O4.2Na 160.036 g/mol
|
0.2 G_PER_L |
CHEBI:115156
disodium fumarate
|
Properties: Simple component, Organic compound, Defined component |
Solutions
Na2CO3 solution
Dose: variable VARIABLE
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1350_Haloarchaeal_Complex_Medium_PH_9.0 (CultureMech:007887)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.407100Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1350 |
| 2026-01-28T19:48:18.765935Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Na2CO3 solution |
| 2026-03-09T08:04:31.135520+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:52.229833Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007887 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.678992+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Haloarchaeal Complex Medium PH 9.0" to "haloarchaeal_complex_medium_ph_9_0" for consistent matching |
| 2026-05-16T09:03:09.585362+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:32.570146+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.659261+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:24.955771+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 1addd263d9429df28f47198d6fd46c5f809ea6bcbc06a79f0269e636e1c7f2b3 |