hydrogenobacter_florentissimum_medium

CultureMech:007888 bacterial COMPLEX LIQUID
Original name: Hydrogenobacter Florentissimum Medium

Overview

External ontology
TOGO:M1351 — Hydrogenobacter Florentissimum Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1351 Original source: JCM - JCM_M1255 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1255

Composition

flowchart LR
    CultureMech_007888["hydrogenobacter_florentissimum_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007888 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007888 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_007888 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007888 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_NH4Cl["NH4Cl"]
    CultureMech_007888 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_KCl["KCl"]
    CultureMech_007888 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_Na2SO4["Na2SO4"]
    CultureMech_007888 --> ing6_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing6_Na2SO4 -.-> CHEBI_32149
    ing7_NaH2PO4["NaH2PO4"]
    CultureMech_007888 --> ing7_NaH2PO4
    CHEBI_37585["CHEBI:37585"]
    ing7_NaH2PO4 -.-> CHEBI_37585
    ing8_NaOH["NaOH"]
    CultureMech_007888 --> ing8_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing8_NaOH -.-> CHEBI_32145
    ing9_N2["N2"]
    CultureMech_007888 --> ing9_N2
    CHEBI_17997["CHEBI:17997"]
    ing9_N2 -.-> CHEBI_17997
    ing10_CO2["CO2"]
    CultureMech_007888 --> ing10_CO2
    CHEBI_16526["CHEBI:16526"]
    ing10_CO2 -.-> CHEBI_16526
    ing11_Nitrogen_gas["Nitrogen gas"]
    CultureMech_007888 --> ing11_Nitrogen_gas
    ing11_Nitrogen_gas -.-> CHEBI_17997
    ing12_H2["H2"]
    CultureMech_007888 --> ing12_H2
    CHEBI_18276["CHEBI:18276"]
    ing12_H2 -.-> CHEBI_18276
    ing13_Air["Air"]
    CultureMech_007888 --> ing13_Air

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.123 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 NaCl
Cl.Na 58.443 g/mol
3 G_PER_L CHEBI:26710
sodium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
4 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.015 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
5 NH4Cl
Cl.H4N 53.492 g/mol
0.107 G_PER_L CHEBI:31206
ammonium chloride
 Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component
6 KCl
Cl.K 74.551 g/mol
0.15 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
7 Na2SO4
2Na.O4S 142.043 g/mol
0.3 G_PER_L CHEBI:32149
sodium sulfate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
8 NaH2PO4
H2O4P.Na 119.976 g/mol
1.38 G_PER_L CHEBI:37585
sodium dihydrogenphosphate
 Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
9 NaOH
HO.Na 39.997 g/mol
variable VARIABLE CHEBI:32145
sodium hydroxide
 Properties: Defined component, Inorganic compound, Solution
10 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas
11 CO2
CO2 44.009 g/mol
variable VARIABLE CHEBI:16526
carbon dioxide
 Properties: Gas
12 Nitrogen gas
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas
13 H2
H2 2.016 g/mol
variable VARIABLE CHEBI:18276
dihydrogen
 Properties: ガス
14 Air variable VARIABLE Properties: ガス

Solutions

Mineral solution (see Medium [M1029])

Dose: 5 G_PER_L

Cross-reference to Medium M1029

10% (w/v) Yeast extract solution

Dose: 10 G_PER_L

Role: Nutrient source; Properties: Undefined component

0.2 M Sodium thiosulfate solution

Dose: 5 G_PER_L

Properties: Defined component, Inorganic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:54.409610Z togo-import Imported from TOGO Medium Source: JCM, ID: M1351
2026-01-28T19:48:25.162102Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: NaOH; Added default concentration for ingredient: N2; Added default concentration for ingredient: CO2; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: H2; Added default concentration for ingredient: Air
2026-03-09T08:04:41.600495+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 3 solution(s) from ingredients
2026-03-13T06:19:52.239723Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007888
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:18.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:10.689003+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Hydrogenobacter Florentissimum Medium" to "hydrogenobacter_florentissimum_medium" for consistent matching
2026-05-16T09:03:09.596618+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:32.581936+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:22.908493+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:47.664226+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:08.217201+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 030defa9670b3135bbf16776ff23031c72bdb8d073711ea176b5af4ac7d89c4b