Overview
- External ontology
- TOGO:M1356 — LSR1 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1356 Original source: JCM - JCM_M1260 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1260
Composition
flowchart LR
CultureMech_007893["lsr1_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007893 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_KH2PO4["KH2PO4"]
CultureMech_007893 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_007893 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_007893 --> ing3_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing3_Na2MoO4_2H2O -.-> CHEBI_75213
ing4_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_007893 --> ing4_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_6H2O -.-> CHEBI_86345
ing5_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_007893 --> ing5_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing5_MnCl2_4H2O -.-> CHEBI_86368
ing6_FeCl3_6H2O["FeCl3・6H2O"]
CultureMech_007893 --> ing6_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing6_FeCl3_6H2O -.-> CHEBI_86254
ing7_K2SO4["K2SO4"]
CultureMech_007893 --> ing7_K2SO4
CHEBI_32036["CHEBI:32036"]
ing7_K2SO4 -.-> CHEBI_32036
ing8_Nitrogen_gas["Nitrogen gas"]
CultureMech_007893 --> ing8_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing8_Nitrogen_gas -.-> CHEBI_17997
ing9_Oxygen_gas["Oxygen gas"]
CultureMech_007893 --> ing9_Oxygen_gas
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component |
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.4 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound |
| 4 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.005 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.12 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 6 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.003 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
FeCl3・6H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.005 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 8 |
K2SO4
2K.O4S 174.259 g/mol
|
0.004 G_PER_L |
CHEBI:32036
potassium sulfate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 9 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 10 | Oxygen gas | variable VARIABLE | Properties: ガス |
Solutions
8% NaHCO3 solution
Dose: 5 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
1.0 M Sodium thiosulfate solution
Dose: 10 G_PER_L
Properties: Defined component, Inorganic compound, Solution
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1356_LSR1_Medium (CultureMech:007893)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.419686Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1356 |
| 2026-01-28T19:48:17.846090Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Oxygen gas |
| 2026-03-09T08:04:29.562516+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:19:52.284585Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007893 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:03.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.736256+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "LSR1 Medium" to "lsr1_medium" for consistent matching |
| 2026-05-16T09:03:09.649994+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:32.640459+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.684990+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:08.270819+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 58985469080e3f7be9e959c811ae537ee81f49aaabbc79b1abebde4c85c76afa |