Overview
- External ontology
- TOGO:M135 — Modified NOS Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M135 Original source: JCM - JCM_M144 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=144 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_007897["modified_nos_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_007897 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Glucose["Glucose"]
CultureMech_007897 --> ing1_Glucose
CHEBI_17234["CHEBI:17234"]
ing1_Glucose -.-> CHEBI_17234
ing2_L__Asparagine["L--Asparagine"]
CultureMech_007897 --> ing2_L__Asparagine
CHEBI_17196["CHEBI:17196"]
ing2_L__Asparagine -.-> CHEBI_17196
ing3_Yeast_extract__BD_BBL_["Yeast extract (BD-BBL)"]
CultureMech_007897 --> ing3_Yeast_extract__BD_BBL_
ing4_Trypticase_peptone__BD_BBL_["Trypticase peptone (BD-BBL)"]
CultureMech_007897 --> ing4_Trypticase_peptone__BD_BBL_
ing5_Sodium_thioglycolate["Sodium thioglycolate"]
CultureMech_007897 --> ing5_Sodium_thioglycolate
CHEBI_86481["CHEBI:86481"]
ing5_Sodium_thioglycolate -.-> CHEBI_86481
ing6_Heart_infusion_broth__BD_Difco_["Heart infusion broth (BD-Difco)"]
CultureMech_007897 --> ing6_Heart_infusion_broth__BD_Difco_
ing7_L__Cysteine_HCl_H2O["L--Cysteine・HCl・H2O"]
CultureMech_007897 --> ing7_L__Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing7_L__Cysteine_HCl_H2O -.-> CHEBI_91248
ing8_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_007897 --> ing8_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing8_Carbon_dioxide_gas -.-> CHEBI_16526
ing9_Nitrogen_gas["Nitrogen gas"]
CultureMech_007897 --> ing9_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing9_Nitrogen_gas -.-> CHEBI_17997
ing10_Hydrogen_gas["Hydrogen gas"]
CultureMech_007897 --> ing10_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing10_Hydrogen_gas -.-> CHEBI_18276
ing11_Rabbit_serum_heat__inactivated_at_56C_for_1_hr["Rabbit serum heat--inactivated at 56C for 1 hr"]
CultureMech_007897 --> ing11_Rabbit_serum_heat__inactivated_at_56C_for_1_hr
ing12_TPP_VFA_mixture__see_below_["TPP/VFA mixture (see below)"]
CultureMech_007897 --> ing12_TPP_VFA_mixture__see_below_
ing13_0_2__Thiamine_pyrophosphate["0.2% Thiamine pyrophosphate"]
CultureMech_007897 --> ing13_0_2__Thiamine_pyrophosphate
CHEBI_9532["CHEBI:9532"]
ing13_0_2__Thiamine_pyrophosphate -.-> CHEBI_9532
ing14_0_1_N_NaOH["0.1 N NaOH"]
CultureMech_007897 --> ing14_0_1_N_NaOH
ing15_Valeric_acid["Valeric acid"]
CultureMech_007897 --> ing15_Valeric_acid
CHEBI_17418["CHEBI:17418"]
ing15_Valeric_acid -.-> CHEBI_17418
ing16_Isovaleric_acid["Isovaleric acid"]
CultureMech_007897 --> ing16_Isovaleric_acid
CHEBI_28484["CHEBI:28484"]
ing16_Isovaleric_acid -.-> CHEBI_28484
ing17_Isobutyric_acid["Isobutyric acid"]
CultureMech_007897 --> ing17_Isobutyric_acid
CHEBI_16135["CHEBI:16135"]
ing17_Isobutyric_acid -.-> CHEBI_16135
ing18_Tryptone["Tryptone"]
CultureMech_007897 --> ing18_Tryptone
ing19_Yeast_extract["Yeast extract"]
CultureMech_007897 --> ing19_Yeast_extract
ing20_Sodium_chloride["Sodium chloride"]
CultureMech_007897 --> ing20_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing20_Sodium_chloride -.-> CHEBI_26710
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
102.4 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 2 duplicates: 94.0, 8.4] |
| 2 |
Glucose
C6H12O6 180.156 g/mol
|
0.2 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 3 |
L--Asparagine
C4H8N2O3 132.119 g/mol
|
0.25 G_PER_L |
CHEBI:17196
L-asparagine
|
Role: Nitrogen source; Properties: Organic compound, Simple component, Defined component |
| 4 | Yeast extract (BD-BBL) | 0.25 G_PER_L | Properties: Undefined component | |
| 5 | Trypticase peptone (BD-BBL) | 1 G_PER_L | Properties: Undefined component | |
| 6 |
Sodium thioglycolate
C2H3O2S.Na 114.101 g/mol
|
0.05 G_PER_L |
CHEBI:86481
sodium thioglycolate
|
Properties: Defined component, Organic compound, Simple component |
| 7 | Heart infusion broth (BD-Difco) | 1.25 G_PER_L | Properties: Complex component, Undefined component | |
| 8 |
L--Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.1 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 9 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 10 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 11 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 12 | Rabbit serum heat--inactivated at 56C for 1 hr | 2 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 13 | TPP/VFA mixture (see below) | 2 G_PER_L | ||
| 14 |
0.2% Thiamine pyrophosphate
C12H19N4O7P2S 425.32 g/mol
|
1.5 G_PER_L |
CHEBI:9532
thiamine(1+) diphosphate
|
Role: Vitamin; Properties: Organic compound, Solution, Defined component |
| 15 | 0.1 N NaOH | 100 G_PER_L | Properties: Defined component, Inorganic compound, Solution | |
| 16 |
Valeric acid
C5H10O2 102.133 g/mol
|
0.5 G_PER_L |
CHEBI:17418
valeric acid
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 17 |
Isovaleric acid
C5H10O2 102.133 g/mol
|
0.5 G_PER_L |
CHEBI:28484
isovaleric acid
|
Role: Carbon source; Properties: Simple component, Organic compound, Defined component |
| 18 |
Isobutyric acid
C4H8O2 88.106 g/mol
|
0.5 G_PER_L |
CHEBI:16135
isobutyric acid
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 19 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 20 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 21 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
Solutions
0.1% Resazurin solution
Dose: 0.1 G_PER_L
Role: pH dependent redox indicator; Properties: Defined component, Solution
7.5% NaHCO3 solution
Dose: 2.67 G_PER_L
Role: Buffer; Properties: Defined component, Inorganic compound, Solution
VFA solution (see below)
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M135_Modified_NOS_Medium (CultureMech:007897)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.404442Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M135 |
| 2026-01-28T19:48:40.272093Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: Hydrogen gas |
| 2026-03-09T08:05:07.875016+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:39.596818Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:52.311917Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007897 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:51.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.765947+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified NOS Medium" to "modified_nos_medium" for consistent matching |
| 2026-05-16T09:03:09.670855+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:32.667832+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:08.301298+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 27958015be289bc49b32d4f64cc4c0dc96c79c95b8fe0d3f8c6a1942986e828b |