modified_picrophilus_medium

CultureMech:007901 archaea COMPLEX LIQUID
Original name: Modified Picrophilus Medium

Overview

External ontology
TOGO:M1363 — Modified Picrophilus Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1363 Original source: JCM - JCM_M1267 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1267

Composition

flowchart LR
    CultureMech_007901["modified_picrophilus_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007901 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_007901 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007901 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3__NH4_2SO4["(NH4)2SO4"]
    CultureMech_007901 --> ing3__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing3__NH4_2SO4 -.-> CHEBI_62946
    ing4_H2SO4["H2SO4"]
    CultureMech_007901 --> ing4_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing4_H2SO4 -.-> CHEBI_26836
    ing5_Biotin["Biotin"]
    CultureMech_007901 --> ing5_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing5_Biotin -.-> CHEBI_15956
    ing6_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_007901 --> ing6_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing6_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing7_Thiamine_HCl["Thiamine-HCl"]
    CultureMech_007901 --> ing7_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing7_Thiamine_HCl -.-> CHEBI_49105
    ing8_Pyridoxine_HCl["Pyridoxine-HCl"]
    CultureMech_007901 --> ing8_Pyridoxine_HCl
    CHEBI_30961["CHEBI:30961"]
    ing8_Pyridoxine_HCl -.-> CHEBI_30961
    ing9_Folic_acid["Folic acid"]
    CultureMech_007901 --> ing9_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing9_Folic_acid -.-> CHEBI_27470
    ing10_Vitamin_B12["Vitamin B12"]
    CultureMech_007901 --> ing10_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing10_Vitamin_B12 -.-> CHEBI_176843
    ing11_Riboflavin["Riboflavin"]
    CultureMech_007901 --> ing11_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing11_Riboflavin -.-> CHEBI_17015
    ing12_Nicotinic_acid["Nicotinic acid"]
    CultureMech_007901 --> ing12_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing12_Nicotinic_acid -.-> CHEBI_15940
    ing13_Nicotine_amide["Nicotine amide"]
    CultureMech_007901 --> ing13_Nicotine_amide
    CHEBI_17154["CHEBI:17154"]
    ing13_Nicotine_amide -.-> CHEBI_17154
    ing14_Lipoic_acid["Lipoic acid"]
    CultureMech_007901 --> ing14_Lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing14_Lipoic_acid -.-> CHEBI_16494
    ing15_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_007901 --> ing15_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing15_DL_Calcium_pantothenate -.-> CHEBI_31345

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
2.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 1.0]
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.25 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.2 G_PER_L CHEBI:62946
ammonium sulfate
 Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
5 H2SO4
H2O4S 98.079 g/mol
variable VARIABLE CHEBI:26836
sulfuric acid
 Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
6 Biotin
C10H16N2O3S 244.316 g/mol
20 G_PER_L CHEBI:15956
biotin
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
7 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
50 G_PER_L CHEBI:30753
4-aminobenzoic acid
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
8 Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
50 G_PER_L CHEBI:49105
thiamine hydrochloride
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
9 Pyridoxine-HCl
C8H11NO3.HCl 205.641 g/mol
10 G_PER_L CHEBI:30961
pyridoxine hydrochloride
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
10 Folic acid
C19H19N7O6 441.404 g/mol
20 G_PER_L CHEBI:27470
folic acid
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
11 Vitamin B12 50 G_PER_L CHEBI:176843
vitamin B12
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
12 Riboflavin
C17H20N4O6 376.369 g/mol
50 G_PER_L CHEBI:17015
riboflavin
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
13 Nicotinic acid
C6H5NO2 123.111 g/mol
25 G_PER_L CHEBI:15940
nicotinic acid
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component
14 Nicotine amide
C6H6N2O 122.127 g/mol
25 G_PER_L CHEBI:17154
nicotinamide
 Role: Growth factor; Properties: Organic compound, Defined component, Simple component
15 Lipoic acid
C8H14O2S2 206.332 g/mol
50 G_PER_L CHEBI:16494
lipoic acid
 Role: Growth factor; Properties: Organic compound, Defined component, Simple component
16 DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
50 G_PER_L CHEBI:31345
Calcium pantothenate
 Role: Vitamin; Properties: Organic compound, Defined component, Simple component

Solutions

10% Yeast extract solution

Dose: 11.1 G_PER_L

Role: Nutrient source; Properties: Undefined component

10% Tryptone solution

Dose: 11.1 G_PER_L

Properties: Undefined component

10% Fe2(SO4)3 solution (in 0.01 N H2SO4 solution)

Dose: 88.8 G_PER_L

Role: Mineral source

Wolfe's mineral elixir (see Medium [M471])

Dose: 2 G_PER_L

Cross-reference to Medium M471

Vitamin solution (see below)

Dose: 2 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:54.432915Z togo-import Imported from TOGO Medium Source: JCM, ID: M1363
2026-01-28T19:48:22.666980Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: H2SO4
2026-03-09T08:04:37.787579+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 5 solution(s) from ingredients
2026-03-13T03:09:07.524999Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:52.349224Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007901
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:30:13.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:10.805629+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified Picrophilus Medium" to "modified_picrophilus_medium" for consistent matching
2026-05-16T09:03:09.703303+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:32.701217+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:30.345776+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T06:57:22.996678+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:47.696490+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:24.971544+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 01e3b69f38069292e2d8be3d0eef1e3a57da90dc99901368830ef2eac5545df2