Overview
- External ontology
- TOGO:M1367 — PHB Agar
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1367 Original source: JCM - JCM_M1271 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1271
Composition
flowchart LR
CultureMech_007905["phb_agar"]
ing0_Distilled_water["Distilled water"]
CultureMech_007905 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_007905 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007905 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_007905 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_007905 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_FeCl3_6H2O["FeCl3・6H2O"]
CultureMech_007905 --> ing5_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing5_FeCl3_6H2O -.-> CHEBI_86254
ing6_Na2HPO4_12H2O["Na2HPO4・12H2O"]
CultureMech_007905 --> ing6_Na2HPO4_12H2O
CHEBI_34683["CHEBI:34683"]
ing6_Na2HPO4_12H2O -.-> CHEBI_34683
ing7_Agar["Agar"]
CultureMech_007905 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
ing8_Poly_3_hydroxybutyrate_["Poly(3-hydroxybutyrate)"]
CultureMech_007905 --> ing8_Poly_3_hydroxybutyrate_
CHEBI_53389["CHEBI:53389"]
ing8_Poly_3_hydroxybutyrate_ -.-> CHEBI_53389
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
4.6 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
FeCl3・6H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.06 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 7 |
Na2HPO4・12H2O
HO4P.2Na 141.958 g/mol
|
11.6 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 8 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 9 |
Poly(3-hydroxybutyrate)
(C4H6O2)n 86.09 g/mol
|
2 G_PER_L |
CHEBI:53389
poly(3-hydroxybutyrate)
|
Role: Carbon source |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1367_PHB_Agar (CultureMech:007905)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.440355Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1367 |
| 2026-01-28T19:48:09.269147Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:52.377363Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007905 |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.834470+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PHB Agar" to "phb_agar" for consistent matching |
| 2026-06-05T07:40:32.724549+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:23.019985+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:47.706662+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:08.352414+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 6a4d49f99bc2e91261c3fb395aef91f6697c94ec5f396539fdd76856c5302402 |