Overview
- External ontology
- TOGO:M1369 — 0.01% LB Seawater Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1369 Original source: JCM - JCM_M1273 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1273 Commercial Product: LB Medium (Luria-Bertani, Miller formulation) Supplier: Multiple suppliers (Difco, Sigma, etc.) Catalog: Multiple (L3522 Sigma, 244520 BD) Source: https://www.laboratorynotes.com/preparation-of-luria-bertani-lb-miller-broth/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_007907["0_01_lb_seawater_medium"]
ing0_K2HPO4["K2HPO4"]
CultureMech_007907 --> ing0_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing0_K2HPO4 -.-> CHEBI_131527
ing1_Biotin["Biotin"]
CultureMech_007907 --> ing1_Biotin
CHEBI_15956["CHEBI:15956"]
ing1_Biotin -.-> CHEBI_15956
ing2_Thiamine_HCl["Thiamine・HCl"]
CultureMech_007907 --> ing2_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing2_Thiamine_HCl -.-> CHEBI_49105
ing3_Vitamin_B12["Vitamin B12"]
CultureMech_007907 --> ing3_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing3_Vitamin_B12 -.-> CHEBI_176843
ing4_NaNO3["NaNO3"]
CultureMech_007907 --> ing4_NaNO3
CHEBI_63005["CHEBI:63005"]
ing4_NaNO3 -.-> CHEBI_63005
ing5_Seawater["Seawater"]
CultureMech_007907 --> ing5_Seawater
ing6_Tris_hydroxymethyl_aminomethane["Tris(hydroxymethyl)aminomethane"]
CultureMech_007907 --> ing6_Tris_hydroxymethyl_aminomethane
CHEBI_9754["CHEBI:9754"]
ing6_Tris_hydroxymethyl_aminomethane -.-> CHEBI_9754
ing7_Fe_III__EDTA["Fe(III)・EDTA"]
CultureMech_007907 --> ing7_Fe_III__EDTA
ing8_Tryptone["Tryptone"]
CultureMech_007907 --> ing8_Tryptone
ing9_Yeast_extract["Yeast extract"]
CultureMech_007907 --> ing9_Yeast_extract
ing10_Sodium_chloride["Sodium chloride"]
CultureMech_007907 --> ing10_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing10_Sodium_chloride -.-> CHEBI_26710
ing11_Mn_II__EDTA["Mn(II)・EDTA"]
CultureMech_007907 --> ing11_Mn_II__EDTA
ing12_HCl["HCl"]
CultureMech_007907 --> ing12_HCl
CHEBI_17883["CHEBI:17883"]
ing12_HCl -.-> CHEBI_17883
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
K2HPO4
HO4P.2K 174.174 g/mol
|
5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
Biotin
C10H16N2O3S 244.316 g/mol
|
1 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Simple component, Organic compound, Defined component |
| 3 |
Thiamine・HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
100 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Simple component, Defined component |
| 4 | Vitamin B12 | 1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Simple component, Defined component |
| 5 |
NaNO3
NO3.Na 84.994 g/mol
|
120 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound |
| 6 | Seawater | 1 G_PER_L | Role: Solvating media, Mineral source; Properties: Undefined component | |
| 7 |
Tris(hydroxymethyl)aminomethane
C4H11NO3 121.136 g/mol
|
1 G_PER_L |
CHEBI:9754
tris
|
Role: Buffer; Properties: Organic compound, Simple component, Defined component |
| 8 | Fe(III)・EDTA | 259 G_PER_L | Properties: Defined component, Organic compound, Simple component | |
| 9 |
Tryptone
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
Pancreatic digest of casein providing amino acids, peptides, and nitrogen; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 10 |
Yeast extract
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
5.0 G_PER_L |
FOODON:03315426
yeast extract
|
Autodigest of Saccharomyces cerevisiae providing vitamins, amino acids, and trace elements; Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 11 |
Sodium chloride
Cl.Na 58.443 g/mol
Multiple suppliers (Difco, Sigma, etc.) LB Medium (Luria-Bertani, Miller formulation) Multiple (L3522 Sigma, 244520 BD)
|
10.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium (Miller formulation); Constituent of LB Medium (Luria-Bertani, Miller formulation) commercial product |
| 12 | Mn(II)・EDTA | 332 G_PER_L | Properties: Defined component, Organic compound, Simple component | |
| 13 |
HCl
HCl 36.461 g/mol
|
variable VARIABLE |
CHEBI:17883
hydrogen chloride
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Solution |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1369_0.01_LB_Seawater_Medium (CultureMech:007907)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.443716Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1369 |
| 2026-01-28T19:48:22.726368Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: HCl |
| 2026-03-13T06:19:52.392874Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007907 |
| 2026-03-17T06:30:13.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.850354+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "0.01% LB Seawater Medium" to "0_01_lb_seawater_medium" for consistent matching |
| 2026-05-16T09:03:09.741217+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:32.743908+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.714271+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:08.375199+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint c6dadec38de0266e1f285e0cf68a292de9f21a1c28cd0c86d025f9e20e467dbf |