Overview
- External ontology
- TOGO:M1375 — MIZ14 Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1375 Original source: JCM - JCM_M1279 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1279
Composition
flowchart LR
CultureMech_007914["miz14_medium"]
ing0_NaHCO3["NaHCO3"]
CultureMech_007914 --> ing0_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing0_NaHCO3 -.-> CHEBI_32139
ing1_Sodium_acetate["Sodium acetate"]
CultureMech_007914 --> ing1_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing1_Sodium_acetate -.-> CHEBI_32954
ing2_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_007914 --> ing2_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing2_Sodium_pyruvate -.-> CHEBI_50144
ing3_MES["MES"]
CultureMech_007914 --> ing3_MES
CHEBI_39010["CHEBI:39010"]
ing3_MES -.-> CHEBI_39010
ing4_N2["N2"]
CultureMech_007914 --> ing4_N2
CHEBI_17997["CHEBI:17997"]
ing4_N2 -.-> CHEBI_17997
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.83 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Simple component, Inorganic compound, Defined component |
| 2 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
1.36 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component |
| 3 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
1.1 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 4 | MES | 1.95 G_PER_L |
CHEBI:39010
MES
|
Role: Buffer; Properties: Defined component, Simple component, Organic compound |
| 5 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Solutions
Modified Wolfe's solution (see Medium [M641])
Dose: 1 G_PER_L
Cross-reference to Medium M641
Trace minerals (see Medium [M142])
Dose: 10 G_PER_L
Cross-reference to Medium M142
10% (w/v) Yeast extract solution
Dose: 10 G_PER_L
Role: Nutrient source; Properties: Undefined component
5% L-Cysteine・HCl・H2O solution
Dose: 10 G_PER_L
Properties: Defined component, Organic compound, Solution
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1375_MIZ14_Medium (CultureMech:007914)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.452693Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1375 |
| 2026-01-28T19:48:31.913930Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:53.139214+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 5 solution(s) from ingredients |
| 2026-03-13T06:19:52.439463Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007914 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:33.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.900099+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MIZ14 Medium" to "miz14_medium" for consistent matching |
| 2026-05-16T09:03:09.795576+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:32.796696+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:08.430020+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 5b9796b40c0043476204bbb325fa4e1acbf05b81d9206671de548f1c1826cb0e |