Overview
- External ontology
- TOGO:M1387 — GS2 Medium With Cellobiose
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1387 Original source: JCM - JCM_M1291 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1291
Composition
flowchart LR
CultureMech_007921["gs2_medium_with_cellobiose"]
ing0_Distilled_water["Distilled water"]
CultureMech_007921 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_007921 --> ing1_Yeast_extract
ing2_KH2PO4["KH2PO4"]
CultureMech_007921 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_K2HPO4["K2HPO4"]
CultureMech_007921 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_Resazurin["Resazurin"]
CultureMech_007921 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_MOPS["MOPS"]
CultureMech_007921 --> ing5_MOPS
CHEBI_39074["CHEBI:39074"]
ing5_MOPS -.-> CHEBI_39074
ing6_Trisodium_citrate_2H2O["Trisodium citrate・2H2O"]
CultureMech_007921 --> ing6_Trisodium_citrate_2H2O
CHEBI_53258["CHEBI:53258"]
ing6_Trisodium_citrate_2H2O -.-> CHEBI_53258
ing7_Urea["Urea"]
CultureMech_007921 --> ing7_Urea
CHEBI_16199["CHEBI:16199"]
ing7_Urea -.-> CHEBI_16199
ing8_L_Cysteine_HCl_H2O["L-Cysteine・HCl・H2O"]
CultureMech_007921 --> ing8_L_Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_H2O -.-> CHEBI_91248
ing9_NaOH["NaOH"]
CultureMech_007921 --> ing9_NaOH
CHEBI_32145["CHEBI:32145"]
ing9_NaOH -.-> CHEBI_32145
ing10_N2["N2"]
CultureMech_007921 --> ing10_N2
CHEBI_17997["CHEBI:17997"]
ing10_N2 -.-> CHEBI_17997
ing11_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_007921 --> ing11_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing11_CaCl2_2H2O -.-> CHEBI_86158
ing12_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_007921 --> ing12_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing12_MgCl2_6H2O -.-> CHEBI_86345
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
2.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 2 duplicates: 1.0, 1.0] |
| 2 | Yeast extract | 6 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.9 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component |
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component |
| 6 | MOPS | 10 G_PER_L |
CHEBI:39074
MOPS
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component |
| 7 |
Trisodium citrate・2H2O
C6H5O7.3Na 258.069 g/mol
|
2.9 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Organic compound, Simple component, Defined component |
| 8 |
Urea
CH4N2O 60.056 g/mol
|
2.1 G_PER_L |
CHEBI:16199
urea
|
Properties: Defined component, Organic compound, Simple component |
| 9 |
L-Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.25 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 10 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 11 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 12 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
15 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 13 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
100 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
Solutions
0.2 M Cellobiose solution
Dose: 8 G_PER_L
Role: Carbon source; Properties: Defined component, Solution, Organic compound
GS2 salt solution (see below)
Dose: 10 G_PER_L
1.25% FeSO4 solution
Dose: 0.1 G_PER_L
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1387_GS2_Medium_With_Cellobiose (CultureMech:007921)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.466545Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1387 |
| 2026-01-28T19:48:33.002133Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:54.639199+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:26.149925Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:52.497027Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007921 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:35.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:10.959003+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "GS2 Medium With Cellobiose" to "gs2_medium_with_cellobiose" for consistent matching |
| 2026-05-16T09:03:09.860194+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:32.864112+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.750261+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:08.517469+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9e3d8deac39bec5adc2ad8322358bd631140857e76a85a3f312f22a5d1455517 |