Overview
- External ontology
- TOGO:M1391 — Alkaline Clostridial Nutrient Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1391 Original source: JCM - JCM_M1295 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1295
Composition
flowchart LR
CultureMech_007926["alkaline_clostridial_nutrient_medium"]
ing0_N2["N2"]
CultureMech_007926 --> ing0_N2
CHEBI_17997["CHEBI:17997"]
ing0_N2 -.-> CHEBI_17997
ing1_Distilled_water["Distilled water"]
CultureMech_007926 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_Yeast_extract["Yeast extract"]
CultureMech_007926 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_007926 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_Sodium_acetate["Sodium acetate"]
CultureMech_007926 --> ing4_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing4_Sodium_acetate -.-> CHEBI_32954
ing5_Glucose["Glucose"]
CultureMech_007926 --> ing5_Glucose
CHEBI_17234["CHEBI:17234"]
ing5_Glucose -.-> CHEBI_17234
ing6_Soluble_starch["Soluble starch"]
CultureMech_007926 --> ing6_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing6_Soluble_starch -.-> CHEBI_28017
ing7_Beef_extract["Beef extract"]
CultureMech_007926 --> ing7_Beef_extract
ing8_L_Cysteine_HCl_H2O["L-Cysteine・HCl・H2O"]
CultureMech_007926 --> ing8_L_Cysteine_HCl_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_H2O -.-> CHEBI_91248
ing9_Peptone["Peptone"]
CultureMech_007926 --> ing9_Peptone
ing10_NaHCO3["NaHCO3"]
CultureMech_007926 --> ing10_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing10_NaHCO3 -.-> CHEBI_32139
ing11_Na2CO3["Na2CO3"]
CultureMech_007926 --> ing11_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing11_Na2CO3 -.-> CHEBI_29377
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 2 |
Distilled water
H2O 18.015 g/mol
|
1000.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 900.0, 100.0] |
| 3 | Yeast extract | 3 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 5 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
3 G_PER_L |
CHEBI:32954
sodium acetate
|
Role: Buffer; Properties: Defined component, Simple component, Organic compound |
| 6 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Simple component, Defined component, Organic compound |
| 7 | Soluble starch | 1 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 8 | Beef extract | 10 G_PER_L | Properties: Undefined component | |
| 9 |
L-Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.5 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
Properties: Defined component, Simple component, Organic compound |
| 10 | Peptone | 5 G_PER_L | ||
| 11 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.9 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 12 |
Na2CO3
CO3.2Na 105.988 g/mol
|
7 G_PER_L |
CHEBI:29377
sodium carbonate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
Solutions
Solution A
Dose: 900 G_PER_L
See mediadive_5342_Solution_A.yaml for composition
Solution B
Dose: 100 G_PER_L
See mediadive_5343_Solution_B.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1391_Alkaline_Clostridial_Nutrient_Medium (CultureMech:007926)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.476612Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M1391 |
| 2026-01-28T19:48:25.908972Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:42.969348+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:09:13.047690Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:52.541777Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007926 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:20.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:11.004136+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Alkaline Clostridial Nutrient Medium" to "alkaline_clostridial_nutrient_medium" for consistent matching |
| 2026-05-16T09:03:09.904556+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:32.909668+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.769044+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:08.568698+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 95a0684e4d0416fb4609ff3e786a4efcaafb2f1561e5b94d1b0ab8b98e99466f |