paenibacillus_dc_8a_medium

CultureMech:007931 bacterial COMPLEX LIQUID
Original name: Paenibacillus DC-8A Medium

Overview

External ontology
TOGO:M1396 — Paenibacillus DC-8A Medium
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M1396 Original source: JCM - JCM_M1299 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=1299

Composition

flowchart LR
    CultureMech_007931["paenibacillus_dc_8a_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007931 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_007931 --> ing1_Yeast_extract
    ing2_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_007931 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_K2HPO4["K2HPO4"]
    CultureMech_007931 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_Resazurin["Resazurin"]
    CultureMech_007931 --> ing4_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Resazurin -.-> CHEBI_8806
    ing5_Na2CO3["Na2CO3"]
    CultureMech_007931 --> ing5_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing5_Na2CO3 -.-> CHEBI_29377
    ing6_Urea["Urea"]
    CultureMech_007931 --> ing6_Urea
    CHEBI_16199["CHEBI:16199"]
    ing6_Urea -.-> CHEBI_16199
    ing7_L_Cysteine_HCl_H2O["L-Cysteine・HCl・H2O"]
    CultureMech_007931 --> ing7_L_Cysteine_HCl_H2O
    CHEBI_91248["CHEBI:91248"]
    ing7_L_Cysteine_HCl_H2O -.-> CHEBI_91248
    ing8_N2["N2"]
    CultureMech_007931 --> ing8_N2
    CHEBI_17997["CHEBI:17997"]
    ing8_N2 -.-> CHEBI_17997
    ing9_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_007931 --> ing9_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing9_MgCl2_6H2O -.-> CHEBI_86345
    ing10_FeSO4_7H2O["FeSO4・7H2O"]
    CultureMech_007931 --> ing10_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing10_FeSO4_7H2O -.-> CHEBI_75836

Ingredients (11)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
901.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 2 duplicates: 900.0, 1.0]
2 Yeast extract 2 G_PER_L Role: Nutrient source; Properties: Undefined component
3 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
37.51 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component [Merged 2 duplicates: 0.01, 37.5]
4 K2HPO4
HO4P.2K 174.174 g/mol
2.9 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
5 Resazurin
C12H7NO4 229.191 g/mol
0.5 G_PER_L CHEBI:8806
Resazurin
 Role: pH dependent redox indicator; Properties: Defined component, Organic compound, Simple component
6 Na2CO3
CO3.2Na 105.988 g/mol
1 G_PER_L CHEBI:29377
sodium carbonate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
7 Urea
CH4N2O 60.056 g/mol
4.2 G_PER_L CHEBI:16199
urea
 Properties: Defined component, Organic compound, Simple component
8 L-Cysteine・HCl・H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
 Properties: Defined component, Simple component, Organic compound
9 N2
N2 28.014 g/mol
variable VARIABLE CHEBI:17997
dinitrogen
 Properties: Gas
10 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
25 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
11 FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
0.312 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component

Solutions

Mineral solution (see below)

Dose: 0.2 G_PER_L

10% (w/v) Xylose solution

Dose: 100 G_PER_L

Role: Carbon source

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:54.485925Z togo-import Imported from TOGO Medium Source: JCM, ID: M1396
2026-01-28T19:48:07.105261Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: N2
2026-03-09T08:04:11.456610+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T03:08:38.181946Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:52.582238Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007931
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:29:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:11.046333+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Paenibacillus DC-8A Medium" to "paenibacillus_dc_8a_medium" for consistent matching
2026-05-16T09:03:09.948940+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:32.957658+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:47.783708+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:08.622666+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint ec8b995dea2a45ab372241b05b5939a000806bf143dcaba03a9a6e35e8c687ba