medium_for_the_2_mapping_strains

CultureMech:007975 bacterial COMPLEX SOLID_AGAR
Original name: Medium for the 2μ Mapping Strains

Overview

External ontology
TOGO:M1436 — Medium for the 2μ Mapping Strains
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1436 Original source: NBRC - NBRC_M114 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=114

Composition

flowchart LR
    CultureMech_007975["medium_for_the_2_mapping_strains"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_007975 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_L_Leucine["L-Leucine"]
    CultureMech_007975 --> ing1_L_Leucine
    CHEBI_15603["CHEBI:15603"]
    ing1_L_Leucine -.-> CHEBI_15603
    ing2_L_Tryptophan["L-Tryptophan"]
    CultureMech_007975 --> ing2_L_Tryptophan
    CHEBI_16828["CHEBI:16828"]
    ing2_L_Tryptophan -.-> CHEBI_16828
    ing3_Glucose["Glucose"]
    CultureMech_007975 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_L_Histidine["L-Histidine"]
    CultureMech_007975 --> ing4_L_Histidine
    CHEBI_15971["CHEBI:15971"]
    ing4_L_Histidine -.-> CHEBI_15971
    ing5_Agar["Agar"]
    CultureMech_007975 --> ing5_Agar
    CHEBI_2509["CHEBI:2509"]
    ing5_Agar -.-> CHEBI_2509
    ing6_L_Methionine["L-Methionine"]
    CultureMech_007975 --> ing6_L_Methionine
    CHEBI_16643["CHEBI:16643"]
    ing6_L_Methionine -.-> CHEBI_16643
    ing7_Bacto_Yeast_Nitrogen_Base_w_o_Amino_acids__Difco_["Bacto-Yeast Nitrogen Base w/o Amino acids (Difco)"]
    CultureMech_007975 --> ing7_Bacto_Yeast_Nitrogen_Base_w_o_Amino_acids__Difco_

Ingredients (8)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 L-Leucine
C6H13NO2 131.175 g/mol
60 G_PER_L CHEBI:15603
L-leucine
Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component
3 L-Tryptophan
C11H12N2O2 204.229 g/mol
20 G_PER_L CHEBI:16828
L-tryptophan
Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component
4 Glucose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:17234
glucose
Role: Carbon source; Properties: Defined component, Organic compound, Simple component
5 L-Histidine
C6H9N3O2 155.157 g/mol
20 G_PER_L CHEBI:15971
L-histidine
Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component
6 Agar 15 G_PER_L CHEBI:2509
agar
Role: Solidifying component
7 L-Methionine
C5H11NO2S 149.215 g/mol
20 G_PER_L CHEBI:16643
L-methionine
Role: Nitrogen source
8 Bacto-Yeast Nitrogen Base w/o Amino acids (Difco) 6.7 G_PER_L Properties: Defined component, Complex component

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:57.675939Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1436
2026-01-28T19:48:11.927761Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:52.802473Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:007975
2026-03-17T06:29:50.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:11.280983+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Medium for the 2μ Mapping Strains" to "medium_for_the_2_mapping_strains" for consistent matching
2026-06-05T07:41:30.098228+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:44.136764+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 6d1383059743449ded00aaf80aba9a9ef1cce8930177c7d6df5b2bad948c18a2