Overview
- External ontology
- TOGO:M144 — Leeming And Notman Agar
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M144 Original source: JCM - JCM_M153 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=153
Composition
flowchart LR
CultureMech_007990["leeming_and_notman_agar"]
ing0_Distilled_water["Distilled water"]
CultureMech_007990 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Glycerol["Glycerol"]
CultureMech_007990 --> ing1_Glycerol
CHEBI_17754["CHEBI:17754"]
ing1_Glycerol -.-> CHEBI_17754
ing2_Cow_s_milk__whole_fat_["Cow's milk (whole fat)"]
CultureMech_007990 --> ing2_Cow_s_milk__whole_fat_
ing3_Oxgall__BD_Difco_["Oxgall (BD-Difco)"]
CultureMech_007990 --> ing3_Oxgall__BD_Difco_
ing4_Glucose["Glucose"]
CultureMech_007990 --> ing4_Glucose
CHEBI_17234["CHEBI:17234"]
ing4_Glucose -.-> CHEBI_17234
ing5_Agar["Agar"]
CultureMech_007990 --> ing5_Agar
CHEBI_2509["CHEBI:2509"]
ing5_Agar -.-> CHEBI_2509
ing6_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_007990 --> ing6_Yeast_extract__BD_Difco_
ing7_Bacto_peptone__BD_Difco_["Bacto peptone (BD-Difco)"]
CultureMech_007990 --> ing7_Bacto_peptone__BD_Difco_
ing8_Glycerol_monostearate["Glycerol monostearate"]
CultureMech_007990 --> ing8_Glycerol_monostearate
CHEBI_75456["CHEBI:75456"]
ing8_Glycerol_monostearate -.-> CHEBI_75456
ing9_Tween_60["Tween 60"]
CultureMech_007990 --> ing9_Tween_60
CHEBI_53425["CHEBI:53425"]
ing9_Tween_60 -.-> CHEBI_53425
Ingredients (10)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound |
| 2 |
Glycerol
C3H8O3 92.094 g/mol
|
1 G_PER_L |
CHEBI:17754
glycerol
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 3 | Cow's milk (whole fat) | 10 G_PER_L | Role: Nitrogen source; Properties: Undefined component | |
| 4 | Oxgall (BD-Difco) | 8 G_PER_L | Role: Selective agent; Properties: Simple component, Undefined component | |
| 5 |
Glucose
C6H12O6 180.156 g/mol
|
5 G_PER_L |
CHEBI:17234
glucose
|
Role: Carbon source; Properties: Defined component, Simple component, Organic compound |
| 6 | Agar | 12 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 7 | Yeast extract (BD-Difco) | 0.1 G_PER_L | Properties: Undefined component | |
| 8 | Bacto peptone (BD-Difco) | 10 G_PER_L | Properties: Undefined component | |
| 9 |
Glycerol monostearate
C21H42O4 358.563 g/mol
|
0.5 G_PER_L |
CHEBI:75456
2-stearoylglycerol
|
Properties: Defined component, Organic compound, Simple component |
| 10 |
Tween 60
(C2H4O)w.(C2H4O)x.(C2H4O)y.(C2H4O)z.C24H46O6 606.838 g/mol
|
0.5 G_PER_L |
CHEBI:53425
polysorbate 60
|
Properties: Organic compound, Defined component, Simple component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M144_Leeming_And_Notman_Agar (CultureMech:007990)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.513167Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M144 |
| 2026-01-28T19:48:16.773477Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:52.869887Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:007990 |
| 2026-03-17T06:30:01.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:11.354075+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Leeming And Notman Agar" to "leeming_and_notman_agar" for consistent matching |
| 2026-06-05T07:40:33.099831+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:30.386506+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-08-06T03:05:08.755808+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint fe644329c37d8d3bde1b04a6bfc7c7aef98fdd3fd3f6c352f21498d7bdbee4fe |