dubos_salts_medium

CultureMech:008012 bacterial COMPLEX SOLID_AGAR
Original name: Dubos Salts Medium

Overview

External ontology
TOGO:M146 — Dubos Salts Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M146 Original source: JCM - JCM_M155 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=155

Composition

flowchart LR
    CultureMech_008012["dubos_salts_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008012 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_008012 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_K2HPO4["K2HPO4"]
    CultureMech_008012 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_FeSO4_7H2O["FeSO4・7H2O"]
    CultureMech_008012 --> ing3_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing3_FeSO4_7H2O -.-> CHEBI_75836
    ing4_KCl["KCl"]
    CultureMech_008012 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_NaNO3["NaNO3"]
    CultureMech_008012 --> ing5_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing5_NaNO3 -.-> CHEBI_63005
    ing6_Agar["Agar"]
    CultureMech_008012 --> ing6_Agar
    CHEBI_2509["CHEBI:2509"]
    ing6_Agar -.-> CHEBI_2509

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
3 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
Role: Buffer; Properties: Defined component, Inorganic compound, Simple component
4 FeSO4・7H2O
Fe.7H2O.O4S 278.013 g/mol
10 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
5 KCl
Cl.K 74.551 g/mol
0.5 G_PER_L CHEBI:32588
potassium chloride
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
6 NaNO3
NO3.Na 84.994 g/mol
0.5 G_PER_L CHEBI:63005
sodium nitrate
Role: Nitrogen source; Properties: Defined component, Simple component, Inorganic compound
7 Agar 15 G_PER_L CHEBI:2509
agar
Role: Solidifying component

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:54.516206Z togo-import Imported from TOGO Medium Source: JCM, ID: M146
2026-01-28T19:48:11.557684Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:52.979858Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008012
2026-03-17T06:29:49.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:11.476284+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Dubos Salts Medium" to "dubos_salts_medium" for consistent matching
2026-06-05T07:40:33.120122+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:23.318409+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:47.823654+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:08.768768+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint dc0f4a091e001af63784b9feceb9d3355bfbace7cab9f61c76152037e5e29a72