rhamnose_salt_medium

CultureMech:008068 bacterial COMPLEX LIQUID
Original name: Rhamnose Salt Medium

Overview

External ontology
TOGO:M1522 — Rhamnose Salt Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1522 Original source: NBRC - NBRC_M309 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=309

Composition

flowchart LR
    CultureMech_008068["rhamnose_salt_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008068 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_008068 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_008068 --> ing2_Yeast_extract
    ing3_NaCl["NaCl"]
    CultureMech_008068 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_008068 --> ing4_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_2H2O -.-> CHEBI_86158
    ing5_KH2PO4["KH2PO4"]
    CultureMech_008068 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_NH4Cl["NH4Cl"]
    CultureMech_008068 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_K2HPO4["K2HPO4"]
    CultureMech_008068 --> ing7_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing7_K2HPO4 -.-> CHEBI_131527
    ing8_FeSO4_7H2O["FeSO4·7H2O"]
    CultureMech_008068 --> ing8_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing8_FeSO4_7H2O -.-> CHEBI_75836
    ing9_Rhamnose["Rhamnose"]
    CultureMech_008068 --> ing9_Rhamnose

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
0.4 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
3 Yeast extract 3 G_PER_L Role: Nutrient source; Properties: Undefined component
4 NaCl
Cl.Na 58.443 g/mol
30 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
5 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
3 G_PER_L CHEBI:86158
calcium chloride dihydrate
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
6 KH2PO4
H2O4P.K 136.084 g/mol
2.1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
7 NH4Cl
Cl.H4N 53.492 g/mol
2 G_PER_L CHEBI:31206
ammonium chloride
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component
8 K2HPO4
HO4P.2K 174.174 g/mol
2.9 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component
9 FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
10 Rhamnose 10 G_PER_L Role: Carbon source; Properties: Defined component, Simple component, Organic compound

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:57.793188Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1522
2026-01-28T19:48:30.506922Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:53.339012Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008068
2026-03-17T06:30:30.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:11.863173+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Rhamnose Salt Medium" to "rhamnose_salt_medium" for consistent matching
2026-06-05T07:41:44.203116+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:58:31.094417+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:47:10.906124+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:54.611359+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 0cd0669f4d291123b109bda77f0b767638451e12bcc0dd0c19e0f54f2769fea1