Overview
- External ontology
- TOGO:M1569 — MMYS-II Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1569 Original source: NBRC - NBRC_M372 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=372
Composition
flowchart LR
CultureMech_008117["mmys_ii_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008117 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_008117 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_008117 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008117 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_008117 --> ing4_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_6H2O -.-> CHEBI_86345
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_008117 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_Na2S2O3_5H2O["Na2S2O3·5H2O"]
CultureMech_008117 --> ing6_Na2S2O3_5H2O
CHEBI_32150["CHEBI:32150"]
ing6_Na2S2O3_5H2O -.-> CHEBI_32150
ing7_Sodium_DL_malate["Sodium DL-malate"]
CultureMech_008117 --> ing7_Sodium_DL_malate
CHEBI_91260["CHEBI:91260"]
ing7_Sodium_DL_malate -.-> CHEBI_91260
ing8_20_mM_Potassium_phosphate_buffer["20 mM Potassium phosphate buffer"]
CultureMech_008117 --> ing8_20_mM_Potassium_phosphate_buffer
ing9_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008117 --> ing9_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing9_Na2MoO4_2H2O -.-> CHEBI_75213
ing10_H3BO3["H3BO3"]
CultureMech_008117 --> ing10_H3BO3
CHEBI_33118["CHEBI:33118"]
ing10_H3BO3 -.-> CHEBI_33118
ing11_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008117 --> ing11_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing11_MnCl2_4H2O -.-> CHEBI_86368
ing12_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008117 --> ing12_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing12_CoCl2_6H2O -.-> CHEBI_35696
ing13_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_008117 --> ing13_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing13_NiCl2_6H2O -.-> CHEBI_34887
ing14_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_008117 --> ing14_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2_2H2O -.-> CHEBI_86318
ing15_ZnCl2["ZnCl2"]
CultureMech_008117 --> ing15_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing15_ZnCl2 -.-> CHEBI_49976
ing16_FeCl2_4H2O["FeCl2·4H2O"]
CultureMech_008117 --> ing16_FeCl2_4H2O
CHEBI_86249["CHEBI:86249"]
ing16_FeCl2_4H2O -.-> CHEBI_86249
ing17_2Na_EDTA["2Na-EDTA"]
CultureMech_008117 --> ing17_2Na_EDTA
CHEBI_64734["CHEBI:64734"]
ing17_2Na_EDTA -.-> CHEBI_64734
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
501.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 500.0, 1.0] |
| 2 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
8.2 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.2 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
Na2S2O3·5H2O
5H2O.2Na.O3S2 248.186 g/mol
|
0.5 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Inorganic compound, Simple component |
| 8 | Sodium DL-malate | 3.6 G_PER_L |
CHEBI:91260
disodium malate
|
Properties: Simple component, Organic compound, Defined component |
| 9 | 20 mM Potassium phosphate buffer | 500 G_PER_L | Role: Buffer; Properties: Defined component, Solution, Inorganic compound, Complex component | |
| 10 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
36 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
H3BO3
H3BO3 61.833 g/mol
|
62 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
100 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
190 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
24 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
17 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
70 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
FeCl2·4H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
2Na-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
5.2 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
Solutions
Solution I*
Dose: 500 G_PER_L
See mediadive_5335_Solution_I.yaml for composition
Solution II**
Dose: 500 G_PER_L
See mediadive_5336_Solution_II.yaml for composition
Trace element solution SL8***
Dose: 1 G_PER_L
See mediadive_3851_Trace_element_solution_SL8.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1569_MMYS-II_Medium (CultureMech:008117)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:57.851591Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1569 |
| 2026-01-28T19:48:15.611692Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:25.785357+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:08:54.301032Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:53.605095Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008117 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:29:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:12.149245+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMYS-II Medium" to "mmys_ii_medium" for consistent matching |
| 2026-05-16T09:03:10.205289+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:33.274160+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:47.851573+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:09.001085+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 7ab162a71355552b6593bf9d70cff8d2ff47bbfd10ee82883988e0633704e723 |