Overview
- External ontology
- TOGO:M1571 — PPES-II Agar Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1571 Original source: NBRC - NBRC_M374 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=374
Composition
flowchart LR
CultureMech_008120["ppes_ii_agar_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_008120 --> ing0_Yeast_extract
ing1_Agar["Agar"]
CultureMech_008120 --> ing1_Agar
CHEBI_2509["CHEBI:2509"]
ing1_Agar -.-> CHEBI_2509
ing2_Fe_III__EDTA["Fe(III)-EDTA"]
CultureMech_008120 --> ing2_Fe_III__EDTA
CHEBI_30729["CHEBI:30729"]
ing2_Fe_III__EDTA -.-> CHEBI_30729
ing3_Bacto_Proteose_Peptone_No_3__Difco_["Bacto Proteose Peptone No.3 (Difco)"]
CultureMech_008120 --> ing3_Bacto_Proteose_Peptone_No_3__Difco_
ing4_Soytone["Soytone"]
CultureMech_008120 --> ing4_Soytone
ing5_Peptone["Peptone"]
CultureMech_008120 --> ing5_Peptone
ing6_Distilled_water["Distilled water"]
CultureMech_008120 --> ing6_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing6_Distilled_water -.-> CHEBI_15377
ing7_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008120 --> ing7_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_7H2O -.-> CHEBI_31795
ing8_NaCl["NaCl"]
CultureMech_008120 --> ing8_NaCl
CHEBI_26710["CHEBI:26710"]
ing8_NaCl -.-> CHEBI_26710
ing9_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008120 --> ing9_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2_2H2O -.-> CHEBI_86158
ing10_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_008120 --> ing10_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing10_MgCl2_6H2O -.-> CHEBI_86345
ing11_KCl["KCl"]
CultureMech_008120 --> ing11_KCl
CHEBI_32588["CHEBI:32588"]
ing11_KCl -.-> CHEBI_32588
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 2 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Role: Solidifying component |
| 3 |
Fe(III)-EDTA
C10H12N2O8.Fe 344.057 g/mol
|
0.1 G_PER_L |
CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)
|
Properties: Defined component, Organic compound, Simple component |
| 4 | Bacto Proteose Peptone No.3 (Difco) | 1 G_PER_L | Properties: Undefined component | |
| 5 | Soytone | 1 G_PER_L | Properties: Undefined component | |
| 6 | Peptone | 2 G_PER_L | ||
| 7 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 8 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
5.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 9 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 10 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 11 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
10.8 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 12 |
KCl
Cl.K 74.551 g/mol
|
0.7 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
Solutions
Artificial sea water*
Dose: 1 G_PER_L
See mediadive_2170_Artificial_sea_water.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1571_PPES-II_Agar_Medium (CultureMech:008120)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:57.855206Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1571 |
| 2026-01-28T19:48:14.386091Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:23.943125+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T06:19:53.625594Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008120 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:56.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:12.171258+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "PPES-II Agar Medium" to "ppes_ii_agar_medium" for consistent matching |
| 2026-05-16T09:03:10.225600+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:33.295584+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:23.458093+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-08-06T03:05:09.013835+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f3f6f93b17f6b733a708ad043bd17ccbc38165c3c69425042d804932f72990ed |