modified_sp_medium

CultureMech:008132 bacterial COMPLEX SOLID_AGAR
Original name: Modified SP Medium

Overview

External ontology
TOGO:M1582 — Modified SP Medium
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1582 Original source: NBRC - NBRC_M390 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=390

Composition

flowchart LR
    CultureMech_008132["modified_sp_medium"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008132 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_008132 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_008132 --> ing2_Yeast_extract
    ing3_K2HPO4["K2HPO4"]
    CultureMech_008132 --> ing3_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing3_K2HPO4 -.-> CHEBI_131527
    ing4_Sucrose["Sucrose"]
    CultureMech_008132 --> ing4_Sucrose
    CHEBI_17992["CHEBI:17992"]
    ing4_Sucrose -.-> CHEBI_17992
    ing5_Galactose["Galactose"]
    CultureMech_008132 --> ing5_Galactose
    CHEBI_28260["CHEBI:28260"]
    ing5_Galactose -.-> CHEBI_28260
    ing6_Raffinose["Raffinose"]
    CultureMech_008132 --> ing6_Raffinose
    CHEBI_16634["CHEBI:16634"]
    ing6_Raffinose -.-> CHEBI_16634
    ing7_Soluble_starch["Soluble starch"]
    CultureMech_008132 --> ing7_Soluble_starch
    CHEBI_28017["CHEBI:28017"]
    ing7_Soluble_starch -.-> CHEBI_28017
    ing8_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008132 --> ing8_Agar__if_needed_
    ing9_Bacto_Casitone__Difco_["Bacto Casitone (Difco)"]
    CultureMech_008132 --> ing9_Bacto_Casitone__Difco_

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Simple component, Inorganic compound, Defined component
2 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 Yeast extract 1 G_PER_L Role: Nutrient source; Properties: Undefined component
4 K2HPO4
HO4P.2K 174.174 g/mol
0.25 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
5 Sucrose
C12H22O11 342.297 g/mol
1 G_PER_L CHEBI:17992
sucrose
Role: Carbon source; Properties: Simple component, Organic compound, Defined component
6 Galactose
C6H12O6 180.156 g/mol
1 G_PER_L CHEBI:28260
galactose
Role: Carbon source; Properties: Defined component, Organic compound, Simple component
7 Raffinose
C18H32O16 504.438 g/mol
1 G_PER_L CHEBI:16634
raffinose
Role: Carbon source; Properties: Organic compound, Simple component, Defined component
8 Soluble starch 5 G_PER_L CHEBI:28017
starch
Role: Carbon source; Properties: Undefined component
9 Agar (if needed) 15 G_PER_L Role: Solidifying component
10 Bacto Casitone (Difco) 2.5 G_PER_L Properties: Undefined component

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:57.882450Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1582
2026-01-28T19:48:21.714670Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:53.750685Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008132
2026-03-17T06:30:11.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:12.299298+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Modified SP Medium" to "modified_sp_medium" for consistent matching
2026-06-05T07:41:37.766591+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:58:26.779264+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:47:08.947563+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:49.791852+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 0cbff31f06bb4734a35a3c4fc9817269ecd2028c90a86c74b4e0996dc3ce5c4f