acidianus_manzaensis_medium_for_heterotrophic_growth

CultureMech:008184 archaea COMPLEX LIQUID
Original name: Acidianus manzaensis Medium for Heterotrophic Growth

Overview

External ontology
TOGO:M1629 — Acidianus manzaensis Medium for Heterotrophic Growth
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1629 Original source: NBRC - NBRC_M831 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=831

Composition

flowchart LR
    CultureMech_008184["acidianus_manzaensis_medium_for_heterotrophic_growth"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008184 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_008184 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_008184 --> ing2_Yeast_extract
    ing3_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_008184 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_KH2PO4["KH2PO4"]
    CultureMech_008184 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_NaMoO4_2H2O["NaMoO4·2H2O"]
    CultureMech_008184 --> ing5_NaMoO4_2H2O
    CHEBI_75213["CHEBI:75213"]
    ing5_NaMoO4_2H2O -.-> CHEBI_75213
    ing6_FeSO4_7H2O["FeSO4·7H2O"]
    CultureMech_008184 --> ing6_FeSO4_7H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_7H2O -.-> CHEBI_75836
    ing7_KCl["KCl"]
    CultureMech_008184 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_CoCl2_6H2O["CoCl2·6H2O"]
    CultureMech_008184 --> ing8_CoCl2_6H2O
    CHEBI_35696["CHEBI:35696"]
    ing8_CoCl2_6H2O -.-> CHEBI_35696
    ing9_NiCl2_6H2O["NiCl2·6H2O"]
    CultureMech_008184 --> ing9_NiCl2_6H2O
    CHEBI_34887["CHEBI:34887"]
    ing9_NiCl2_6H2O -.-> CHEBI_34887
    ing10_ZnSO4_7H2O["ZnSO4·7H2O"]
    CultureMech_008184 --> ing10_ZnSO4_7H2O
    CHEBI_32312["CHEBI:32312"]
    ing10_ZnSO4_7H2O -.-> CHEBI_32312
    ing11_CuSO4_5H2O["CuSO4·5H2O"]
    CultureMech_008184 --> ing11_CuSO4_5H2O
    CHEBI_31440["CHEBI:31440"]
    ing11_CuSO4_5H2O -.-> CHEBI_31440
    ing12_MnSO4_5H2O["MnSO4·5H2O"]
    CultureMech_008184 --> ing12_MnSO4_5H2O
    CHEBI_131524["CHEBI:131524"]
    ing12_MnSO4_5H2O -.-> CHEBI_131524
    ing13__NH4_2SO4_7H2O["(NH4)2SO4·7H2O"]
    CultureMech_008184 --> ing13__NH4_2SO4_7H2O
    ing14_H2SO4["H2SO4"]
    CultureMech_008184 --> ing14_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing14_H2SO4 -.-> CHEBI_26836

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component
2 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
0.49 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3 Yeast extract 1 G_PER_L Role: Nutrient source; Properties: Undefined component
4 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.009 G_PER_L CHEBI:86158
calcium chloride dihydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
5 KH2PO4
H2O4P.K 136.084 g/mol
0.041 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
6 NaMoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
0.5 G_PER_L CHEBI:75213
sodium molybdate dihydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
7 FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
5 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
8 KCl
Cl.K 74.551 g/mol
0.052 G_PER_L CHEBI:32588
potassium chloride
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
9 CoCl2·6H2O
2Cl.Co 129.839 g/mol
0.5 G_PER_L CHEBI:35696
cobalt dichloride
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
10 NiCl2·6H2O
Cl2Ni 129.599 g/mol
1 G_PER_L CHEBI:34887
nickel dichloride
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
11 ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
12 CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
2 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
13 MnSO4·5H2O
5H2O.Mn.O4S 241.076 g/mol
1 G_PER_L CHEBI:131524
manganese(II) sulfate pentahydrate
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
14 (NH4)2SO4·7H2O 0.132 G_PER_L Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
15 H2SO4
H2O4S 98.079 g/mol
variable VARIABLE CHEBI:26836
sulfuric acid
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:58.004053Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1629
2026-01-28T19:48:10.215475Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: H2SO4
2026-03-13T06:19:54.226313Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008184
2026-03-17T06:29:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:12.807476+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Acidianus manzaensis Medium for Heterotrophic Growth" to "acidianus_manzaensis_medium_for_heterotrophic_growth" for consistent matching
2026-05-16T09:03:23.095058+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:47.061715+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:34.941916+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:52.504271+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:28.138265+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint dfdf2ad52939b3ae973d712d2717b17871006b24cfc12672107c26cb05cfd68a