Overview
- External ontology
- TOGO:M162 — Artificial Deep Lake Vitamin Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M162 Original source: JCM - JCM_M170 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=170
Composition
flowchart LR
CultureMech_008185["artificial_deep_lake_vitamin_medium"]
ing0_distilled_water["distilled water"]
CultureMech_008185 --> ing0_distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4・7H2O"]
CultureMech_008185 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_008185 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_008185 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_008185 --> ing4_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_6H2O -.-> CHEBI_86345
ing5_KCl["KCl"]
CultureMech_008185 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_Sodium_succinate["Sodium succinate"]
CultureMech_008185 --> ing6_Sodium_succinate
CHEBI_63675["CHEBI:63675"]
ing6_Sodium_succinate -.-> CHEBI_63675
ing7_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_008185 --> ing7_Yeast_extract__BD_Difco_
ing8_Biotin["Biotin"]
CultureMech_008185 --> ing8_Biotin
CHEBI_15956["CHEBI:15956"]
ing8_Biotin -.-> CHEBI_15956
ing9_Thiamine_HCl["Thiamine・HCl"]
CultureMech_008185 --> ing9_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing9_Thiamine_HCl -.-> CHEBI_49105
ing10_Vitamin_B12["Vitamin B12"]
CultureMech_008185 --> ing10_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing10_Vitamin_B12 -.-> CHEBI_176843
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
distilled water
H2O 18.015 g/mol
|
991.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Simple component, Inorganic compound [Merged 2 duplicates: 990.0, 1.0] |
| 2 |
MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
|
7.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
180 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 5 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
75 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 6 |
KCl
Cl.K 74.551 g/mol
|
7.4 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
Sodium succinate
C4H4O4.2Na 162.052 g/mol
|
10 G_PER_L |
CHEBI:63675
sodium succinate (anhydrous)
|
Role: Growth factor; Properties: Defined component, Simple component, Organic compound |
| 8 | Yeast extract (BD-Difco) | 1 G_PER_L | Properties: Undefined component | |
| 9 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.1 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Simple component, Defined component, Organic compound |
| 10 |
Thiamine・HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.1 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Simple component, Organic compound, Defined component |
| 11 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Defined component, Organic compound, Simple component |
Solutions
Vitamin solution (see below)
Dose: 10 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M162_Artificial_Deep_Lake_Vitamin_Medium (CultureMech:008185)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:54.540269Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M162 |
| 2026-01-28T19:48:15.625282Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:25.810795+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:08:54.326226Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:54.234096Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008185 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:58.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:12.815787+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Artificial Deep Lake Vitamin Medium" to "artificial_deep_lake_vitamin_medium" for consistent matching |
| 2026-05-16T09:03:10.503510+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:33.609573+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:23.761598+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-08-06T03:05:09.228950+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 98d45e3e8b0c901cb5d36e1668ccf870323240dc49e87ec906ffd60211119ffe |