Overview
- External ontology
- TOGO:M1635 — Thermoproteus tenax Medium 2
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1635 Original source: NBRC - NBRC_M838 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=838
Composition
flowchart LR
CultureMech_008190["thermoproteus_tenax_medium_2"]
ing0_Distilled_water["Distilled water"]
CultureMech_008190 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008190 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008190 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_008190 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008190 --> ing4_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing4_Na2MoO4_2H2O -.-> CHEBI_75213
ing5_Resazurin["Resazurin"]
CultureMech_008190 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_Na2S_9H2O["Na2S·9H2O"]
CultureMech_008190 --> ing6_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing6_Na2S_9H2O -.-> CHEBI_76209
ing7_NaHCO3["NaHCO3"]
CultureMech_008190 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008190 --> ing8_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing8_MnCl2_4H2O -.-> CHEBI_86368
ing9_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008190 --> ing9_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing9_ZnSO4_7H2O -.-> CHEBI_32312
ing10_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_008190 --> ing10_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing10_CuCl2_2H2O -.-> CHEBI_86318
ing11__NH4_2SO4["(NH4)2SO4"]
CultureMech_008190 --> ing11__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing11__NH4_2SO4 -.-> CHEBI_62946
ing12_FeCl3_6H2O["FeCl3·6H2O"]
CultureMech_008190 --> ing12_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing12_FeCl3_6H2O -.-> CHEBI_86254
ing13_CoSO4_7H2O["CoSO4·7H2O"]
CultureMech_008190 --> ing13_CoSO4_7H2O
CHEBI_91244["CHEBI:91244"]
ing13_CoSO4_7H2O -.-> CHEBI_91244
ing14_Sulfur__powder_["Sulfur (powder)"]
CultureMech_008190 --> ing14_Sulfur__powder_
CHEBI_33403["CHEBI:33403"]
ing14_Sulfur__powder_ -.-> CHEBI_33403
ing15_VOSO4_xH2O["VOSO4·xH2O"]
CultureMech_008190 --> ing15_VOSO4_xH2O
CHEBI_87020["CHEBI:87020"]
ing15_VOSO4_xH2O -.-> CHEBI_87020
ing16_Na2B4O7_10H2O["Na2B4O7·10H2O"]
CultureMech_008190 --> ing16_Na2B4O7_10H2O
CHEBI_131366["CHEBI:131366"]
ing16_Na2B4O7_10H2O -.-> CHEBI_131366
ing17_Soluble_starch["Soluble starch"]
CultureMech_008190 --> ing17_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing17_Soluble_starch -.-> CHEBI_28017
ing18_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_008190 --> ing18_Bacto_Yeast_Extract__Difco_
ing19_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_008190 --> ing19_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing19_Carbon_dioxide_gas -.-> CHEBI_16526
ing20_Nitrogen_gas["Nitrogen gas"]
CultureMech_008190 --> ing20_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing20_Nitrogen_gas -.-> CHEBI_17997
Ingredients (21)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.07 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.28 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 7 |
Na2S·9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.85 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.8 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.22 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.05 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
FeCl3·6H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.02 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
CoSO4·7H2O
Co.7H2O.O4S 281.101 g/mol
|
0.01 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 | Sulfur (powder) | 8 G_PER_L |
CHEBI:33403
elemental sulfur
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
VOSO4·xH2O
(H2O)n.O5SV 181.021 g/mol
|
0.03 G_PER_L |
CHEBI:87020
vanadyl sulfate hydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
Na2B4O7·10H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
4.5 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 | Soluble starch | 5 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 19 | Bacto Yeast Extract (Difco) | 0.2 G_PER_L | Properties: Undefined component | |
| 20 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 21 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- thermoproteus_tenax_medium_2 (CultureMech:008190)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:58.020788Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1635 |
| 2026-01-28T19:48:31.498923Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-13T06:19:54.308699Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008190 |
| 2026-03-17T06:30:32.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:12.892165+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Thermoproteus tenax Medium 2" to "thermoproteus_tenax_medium_2" for consistent matching |
| 2026-05-16T09:04:34.851184+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:50.485198+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:35.419556+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:13.142088+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:33.037015+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 7c249975cb8e74d0aa04c6a016bdbfe722b4902a8593283117f2b1a172927c54 |