Overview
- External ontology
- TOGO:M1639 — Mycoplasma Medium with Sucrose
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1639 Original source: NBRC - NBRC_M842 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=842
Composition
flowchart LR
CultureMech_008194["mycoplasma_medium_with_sucrose"]
ing0_Distilled_water["Distilled water"]
CultureMech_008194 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Sucrose["Sucrose"]
CultureMech_008194 --> ing1_Sucrose
CHEBI_17992["CHEBI:17992"]
ing1_Sucrose -.-> CHEBI_17992
ing2_Phenol_red["Phenol red"]
CultureMech_008194 --> ing2_Phenol_red
CHEBI_31991["CHEBI:31991"]
ing2_Phenol_red -.-> CHEBI_31991
ing3_Penicillin_G["Penicillin G"]
CultureMech_008194 --> ing3_Penicillin_G
ing4_Bacto_Heart_Infusion_Broth__Difco_["Bacto Heart Infusion Broth (Difco)"]
CultureMech_008194 --> ing4_Bacto_Heart_Infusion_Broth__Difco_
Ingredients (5)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
650 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
Sucrose
C12H22O11 342.297 g/mol
|
40 G_PER_L |
CHEBI:17992
sucrose
|
Role: Carbon source; Properties: Simple component, Defined component, Organic compound |
| 3 |
Phenol red
C19H14O5S 354.383 g/mol
|
40 G_PER_L |
CHEBI:31991
phenol red
|
Role: pH indicator; Properties: Organic compound, Simple component, Defined component |
| 4 | Penicillin G | 1 G_PER_L | Role: Antimicrobial agent; Properties: Simple component, Defined component | |
| 5 | Bacto Heart Infusion Broth (Difco) | 17.5 G_PER_L | Properties: Undefined component, Complex component |
Solutions
15% Yeast Extract Solution (Gibco 0689)
Dose: 150 G_PER_L
Properties: Undefined component
Horse serum*
Dose: 200 G_PER_L
Role: Nutrient source; Properties: Undefined component
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mycoplasma_medium_with_sucrose (CultureMech:008194)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.030939Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1639 |
| 2026-01-28T19:48:42.934761Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:05:12.372991+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:19:54.347965Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008194 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:12.933645+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Mycoplasma Medium with Sucrose" to "mycoplasma_medium_with_sucrose" for consistent matching |
| 2026-05-16T09:04:27.172643+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:39.046498+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:50.940007+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint e84dcd8420e4ee9007d934614cf74b6d032302fd05e7fd22bde1ad33ad7d9572 |