artificial_deep_lake_vitamin_medium

CultureMech:008195 bacterial COMPLEX SOLID_AGAR
Original name: Artificial Deep Lake Vitamin Medium

Overview

External ontology
TOGO:M163 — Artificial Deep Lake Vitamin Medium
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: https://togomedium.org/medium/M163 Original source: JCM - JCM_M170-2 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=170

Composition

flowchart LR
    CultureMech_008195["artificial_deep_lake_vitamin_medium"]
    ing0_distilled_water["distilled water"]
    CultureMech_008195 --> ing0_distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4・7H2O"]
    CultureMech_008195 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_NaCl["NaCl"]
    CultureMech_008195 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_CaCl2_2H2O["CaCl2・2H2O"]
    CultureMech_008195 --> ing3_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing3_CaCl2_2H2O -.-> CHEBI_86158
    ing4_MgCl2_6H2O["MgCl2・6H2O"]
    CultureMech_008195 --> ing4_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing4_MgCl2_6H2O -.-> CHEBI_86345
    ing5_KCl["KCl"]
    CultureMech_008195 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_Sodium_succinate["Sodium succinate"]
    CultureMech_008195 --> ing6_Sodium_succinate
    CHEBI_63675["CHEBI:63675"]
    ing6_Sodium_succinate -.-> CHEBI_63675
    ing7_Noble_Agar__BD_Difco_["Noble Agar (BD-Difco)"]
    CultureMech_008195 --> ing7_Noble_Agar__BD_Difco_
    ing8_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
    CultureMech_008195 --> ing8_Yeast_extract__BD_Difco_

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 distilled water
H2O 18.015 g/mol
990 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4・7H2O
7H2O.Mg.O4S 246.473 g/mol
7.4 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
3 NaCl
Cl.Na 58.443 g/mol
180 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
4 CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
1 G_PER_L CHEBI:86158
calcium chloride dihydrate
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
5 MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
75 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
6 KCl
Cl.K 74.551 g/mol
7.4 G_PER_L CHEBI:32588
potassium chloride
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
7 Sodium succinate
C4H4O4.2Na 162.052 g/mol
10 G_PER_L CHEBI:63675
sodium succinate (anhydrous)
Role: Growth factor; Properties: Simple component, Defined component, Organic compound
8 Noble Agar (BD-Difco) 20 G_PER_L Role: Solidifying component; Properties: Defined component
9 Yeast extract (BD-Difco) 1 G_PER_L Properties: Undefined component

Solutions

Vitamin solution (see Medium [M162])

Dose: 10 G_PER_L

Cross-reference to Medium M162

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:54.542030Z togo-import Imported from TOGO Medium Source: JCM, ID: M163
2026-01-28T19:48:26.776556Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:44.420183+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:54.353611Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008195
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:22.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:12.939934+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Artificial Deep Lake Vitamin Medium" to "artificial_deep_lake_vitamin_medium" for consistent matching
2026-05-16T09:03:10.597572+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:33.702760+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:23.838921+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-08-06T03:05:09.246347+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint be730e7aa014b21b43698d60dbe2b977212e8b4c0c22b663c5996814b5d11c34