byg_medium_for_acidiphilium_multivorum

CultureMech:008199 bacterial COMPLEX SOLID_AGAR
Original name: BYG Medium (for Acidiphilium multivorum)

Overview

External ontology
TOGO:M1643 — BYG Medium (for Acidiphilium multivorum)
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1643 Original source: NBRC - NBRC_M846 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=846

Composition

flowchart LR
    CultureMech_008199["byg_medium_for_acidiphilium_multivorum"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008199 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_008199 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_008199 --> ing2_Yeast_extract
    ing3_KH2PO4["KH2PO4"]
    CultureMech_008199 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_KCl["KCl"]
    CultureMech_008199 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5__NH4_2SO4["(NH4)2SO4"]
    CultureMech_008199 --> ing5__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing5__NH4_2SO4 -.-> CHEBI_62946
    ing6_Ca_NO3_2_4H2O["Ca(NO3)2·4H2O"]
    CultureMech_008199 --> ing6_Ca_NO3_2_4H2O
    CHEBI_86159["CHEBI:86159"]
    ing6_Ca_NO3_2_4H2O -.-> CHEBI_86159
    ing7_H2SO4["H2SO4"]
    CultureMech_008199 --> ing7_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing7_H2SO4 -.-> CHEBI_26836
    ing8_Glucose["Glucose"]
    CultureMech_008199 --> ing8_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing8_Glucose -.-> CHEBI_17234
    ing9_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008199 --> ing9_Agar__if_needed_

Ingredients (10)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1000.0 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Simple component, Defined component, Inorganic compound [Merged 2 duplicates: 500.0, 500.0]
2 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
3 Yeast extract 0.1 G_PER_L Role: Nutrient source; Properties: Undefined component
4 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Simple component, Defined component, Inorganic compound
5 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
6 (NH4)2SO4
2H4N.O4S 132.141 g/mol
2 G_PER_L CHEBI:62946
ammonium sulfate
 Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component
7 Ca(NO3)2·4H2O
Ca.4H2O.2NO3 236.146 g/mol
0.01 G_PER_L CHEBI:86159
calcium nitrate tetrahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
8 H2SO4
H2O4S 98.079 g/mol
variable VARIABLE CHEBI:26836
sulfuric acid
 Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
9 Glucose
C6H12O6 180.156 g/mol
1 G_PER_L CHEBI:17234
glucose
 Role: Carbon source; Properties: Defined component, Organic compound, Simple component
10 Agar (if needed) 20 G_PER_L Role: Solidifying component

Solutions

Solution A*

Dose: 500 G_PER_L

See mediadive_5342_Solution_A.yaml for composition

Solution B**

Dose: 500 G_PER_L

See mediadive_5343_Solution_B.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:58.036905Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1643
2026-01-28T19:48:41.134933Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: H2SO4
2026-03-09T08:05:09.250387+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 2 solution(s) from ingredients
2026-03-13T03:09:41.010419Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:54.382188Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008199
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name; Added default solution name
2026-03-17T06:30:53.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:12.971605+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "BYG Medium (for Acidiphilium multivorum)" to "byg_medium_for_acidiphilium_multivorum" for consistent matching
2026-05-16T09:03:26.386322+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=2
2026-06-05T07:40:51.908801+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:57:38.185216+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:46:54.042056+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:26.198672+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint f08bb61117aca48b617fcfe2e72a65e240f6dc8a01df8487915ce0dfef4140a7