Overview
- External ontology
- TOGO:M1647 — Modified MB Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1647 Original source: NBRC - NBRC_M850 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=850
Composition
flowchart LR
CultureMech_008203["modified_mb_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008203 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_008203 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_008203 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008203 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_008203 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_008203 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_K2HPO4["K2HPO4"]
CultureMech_008203 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_Resazurin["Resazurin"]
CultureMech_008203 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_008203 --> ing8_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing8_MgCl2_6H2O -.-> CHEBI_86345
ing9_KCl["KCl"]
CultureMech_008203 --> ing9_KCl
CHEBI_32588["CHEBI:32588"]
ing9_KCl -.-> CHEBI_32588
ing10_Soluble_starch["Soluble starch"]
CultureMech_008203 --> ing10_Soluble_starch
CHEBI_28017["CHEBI:28017"]
ing10_Soluble_starch -.-> CHEBI_28017
ing11_Cysteine_HCl["Cysteine-HCl"]
CultureMech_008203 --> ing11_Cysteine_HCl
CHEBI_91247["CHEBI:91247"]
ing11_Cysteine_HCl -.-> CHEBI_91247
ing12_Na2S2O3_5H2O["Na2S2O3·5H2O"]
CultureMech_008203 --> ing12_Na2S2O3_5H2O
CHEBI_32150["CHEBI:32150"]
ing12_Na2S2O3_5H2O -.-> CHEBI_32150
ing13_Bacto_Tryptone__Difco_["Bacto Tryptone (Difco)"]
CultureMech_008203 --> ing13_Bacto_Tryptone__Difco_
ing14_NaOH["NaOH"]
CultureMech_008203 --> ing14_NaOH
CHEBI_32145["CHEBI:32145"]
ing14_NaOH -.-> CHEBI_32145
ing15_N2["N2"]
CultureMech_008203 --> ing15_N2
CHEBI_17997["CHEBI:17997"]
ing15_N2 -.-> CHEBI_17997
ing16_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008203 --> ing16_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing16_MgSO4_7H2O -.-> CHEBI_31795
ing17_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008203 --> ing17_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_2H2O -.-> CHEBI_75213
ing18_H3BO3["H3BO3"]
CultureMech_008203 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008203 --> ing19_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing19_FeSO4_7H2O -.-> CHEBI_75836
ing20_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_008203 --> ing20_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing20_NiCl2_6H2O -.-> CHEBI_34887
ing21_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008203 --> ing21_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing21_ZnSO4_7H2O -.-> CHEBI_32312
ing22_CuSO4_5H2O["CuSO4·5H2O"]
CultureMech_008203 --> ing22_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing22_CuSO4_5H2O -.-> CHEBI_31440
ing23_Na2SeO3_5H2O["Na2SeO3·5H2O"]
CultureMech_008203 --> ing23_Na2SeO3_5H2O
CHEBI_131361["CHEBI:131361"]
ing23_Na2SeO3_5H2O -.-> CHEBI_131361
ing24_CoSO4_7H2O["CoSO4·7H2O"]
CultureMech_008203 --> ing24_CoSO4_7H2O
CHEBI_91244["CHEBI:91244"]
ing24_CoSO4_7H2O -.-> CHEBI_91244
ing25_KAl_SO4_2_12H2O["KAl(SO4)2·12H2O"]
CultureMech_008203 --> ing25_KAl_SO4_2_12H2O
CHEBI_86465["CHEBI:86465"]
ing25_KAl_SO4_2_12H2O -.-> CHEBI_86465
ing26_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_008203 --> ing26_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing26_Nitrilotriacetic_acid -.-> CHEBI_44557
ing27_MnSO4_2H2O["MnSO4·2H2O"]
CultureMech_008203 --> ing27_MnSO4_2H2O
CHEBI_86356["CHEBI:86356"]
ing27_MnSO4_2H2O -.-> CHEBI_86356
ing28_KOH["KOH"]
CultureMech_008203 --> ing28_KOH
CHEBI_32035["CHEBI:32035"]
ing28_KOH -.-> CHEBI_32035
Ingredients (29)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
2.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 1.0, 1.0] |
| 2 | Yeast extract | 1 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
3.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 2.0, 1.0] |
| 4 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.15000000000000002 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.05, 0.1] |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.3 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 9 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.5 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
KCl
Cl.K 74.551 g/mol
|
0.2 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 | Soluble starch | 10 G_PER_L |
CHEBI:28017
starch
|
Role: Carbon source; Properties: Undefined component |
| 12 |
Cysteine-HCl
C3H7NO2S.HCl 157.622 g/mol
|
0.5 G_PER_L |
CHEBI:91247
L-cysteine hydrochloride
|
Properties: Defined component, Organic compound, Simple component |
| 13 |
Na2S2O3·5H2O
5H2O.2Na.O3S2 248.186 g/mol
|
5 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Inorganic compound, Simple component |
| 14 | Bacto Tryptone (Difco) | 2 G_PER_L | Properties: Undefined component | |
| 15 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 16 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 17 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
Na2SeO3·5H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.3 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
CoSO4·7H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
KAl(SO4)2·12H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 27 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 28 |
MnSO4·2H2O
2H2O.Mn.O4S 187.031 g/mol
|
0.5 G_PER_L |
CHEBI:86356
manganese(II) sulfate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
Solutions
Trace element solution*
Dose: 10 G_PER_L
See mediadive_6187_Trace_element_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_mb_medium (CultureMech:008203)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.043012Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1647 |
| 2026-01-28T19:48:41.665263Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH; Added default concentration for ingredient: N2; Added default concentration for ingredient: KOH |
| 2026-03-09T08:05:10.290642+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:09:42.070000Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:54.414628Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008203 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:30:54.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:13.005607+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Modified MB Medium" to "modified_mb_medium" for consistent matching |
| 2026-05-16T09:04:25.708638+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:41:37.095738+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:58:26.301875+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:08.724697+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:48.839198+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint fe2a1f2d8489e43d1189d5682853bd0dcc88e0bf1882b934e141fd39be7b8001 |