sulfurisphaera_medium_ph_5_0

CultureMech:008215 archaea COMPLEX LIQUID
Original name: Sulfurisphaera Medium (pH 5.0)

Overview

External ontology
TOGO:M1658 — Sulfurisphaera Medium (pH 5.0)
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'sulfurisphaera_medium_ph_2_5', 'source': 'TOGO', 'source_id': 'TOGO:M1657', 'original_category': 'archaea'}
Notes
Source: https://togomedium.org/medium/M1658 Original source: NBRC - NBRC_M862 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=862

Composition

flowchart LR
    CultureMech_008215["sulfurisphaera_medium_ph_5_0"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008215 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_MgSO4_7H2O["MgSO4·7H2O"]
    CultureMech_008215 --> ing1_MgSO4_7H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_7H2O -.-> CHEBI_31795
    ing2_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_008215 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_008215 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_Na2MoO4_2H2O["Na2MoO4·2H2O"]
    CultureMech_008215 --> ing4_Na2MoO4_2H2O
    CHEBI_75213["CHEBI:75213"]
    ing4_Na2MoO4_2H2O -.-> CHEBI_75213
    ing5_MnCl2_4H2O["MnCl2·4H2O"]
    CultureMech_008215 --> ing5_MnCl2_4H2O
    CHEBI_86368["CHEBI:86368"]
    ing5_MnCl2_4H2O -.-> CHEBI_86368
    ing6_ZnSO4_7H2O["ZnSO4·7H2O"]
    CultureMech_008215 --> ing6_ZnSO4_7H2O
    CHEBI_32312["CHEBI:32312"]
    ing6_ZnSO4_7H2O -.-> CHEBI_32312
    ing7_CuCl2_2H2O["CuCl2·2H2O"]
    CultureMech_008215 --> ing7_CuCl2_2H2O
    CHEBI_86318["CHEBI:86318"]
    ing7_CuCl2_2H2O -.-> CHEBI_86318
    ing8__NH4_2SO4["(NH4)2SO4"]
    CultureMech_008215 --> ing8__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing8__NH4_2SO4 -.-> CHEBI_62946
    ing9_FeCl3_6H2O["FeCl3·6H2O"]
    CultureMech_008215 --> ing9_FeCl3_6H2O
    CHEBI_86254["CHEBI:86254"]
    ing9_FeCl3_6H2O -.-> CHEBI_86254
    ing10_CoSO4_7H2O["CoSO4·7H2O"]
    CultureMech_008215 --> ing10_CoSO4_7H2O
    CHEBI_91244["CHEBI:91244"]
    ing10_CoSO4_7H2O -.-> CHEBI_91244
    ing11_VOSO4_nH2O["VOSO4·nH2O"]
    CultureMech_008215 --> ing11_VOSO4_nH2O
    ing12_Na2B4O7_10H2O["Na2B4O7·10H2O"]
    CultureMech_008215 --> ing12_Na2B4O7_10H2O
    CHEBI_131366["CHEBI:131366"]
    ing12_Na2B4O7_10H2O -.-> CHEBI_131366
    ing13_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
    CultureMech_008215 --> ing13_Bacto_Yeast_Extract__Difco_
    ing14_H2SO4["H2SO4"]
    CultureMech_008215 --> ing14_H2SO4
    CHEBI_26836["CHEBI:26836"]
    ing14_H2SO4 -.-> CHEBI_26836

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
 Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
0.25 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component
3 CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
0.07 G_PER_L CHEBI:86158
calcium chloride dihydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
4 KH2PO4
H2O4P.K 136.084 g/mol
0.28 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
 Role: Buffer; Properties: Defined component, Simple component, Inorganic compound
5 Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
6 MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
1.8 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
7 ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
0.22 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
8 CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
0.05 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
9 (NH4)2SO4
2H4N.O4S 132.141 g/mol
1.3 G_PER_L CHEBI:62946
ammonium sulfate
 Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component
10 FeCl3·6H2O
Cl3Fe.6H2O 270.294 g/mol
0.02 G_PER_L CHEBI:86254
iron trichloride hexahydrate
 Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
11 CoSO4·7H2O
Co.7H2O.O4S 281.101 g/mol
0.01 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
 Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component
12 VOSO4·nH2O 0.03 G_PER_L Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound
13 Na2B4O7·10H2O
10H2O.H4B4O9.2Na 417.401 g/mol
4.5 G_PER_L CHEBI:131366
disodium tetraborate decahydrate
 Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound
14 Bacto Yeast Extract (Difco) 1 G_PER_L Properties: Undefined component
15 H2SO4
H2O4S 98.079 g/mol
variable VARIABLE CHEBI:26836
sulfuric acid
 Role: Mineral source; Properties: Defined component, Inorganic compound, Solution

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-27T06:35:58.079465Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1658
2026-01-28T19:48:28.969812Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: H2SO4
2026-03-13T06:19:54.576867Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008215
2026-03-17T06:30:26.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:13.177648+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "Sulfurisphaera Medium (pH 5.0)" to "sulfurisphaera_medium_ph_5_0" for consistent matching
2026-05-16T09:04:33.102798+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:47.885014+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T06:58:33.658886+00:00 mgso4-heptahydrate-term-fix-v1.0 Corrected MgSO4·7H2O primary term grounding to heptahydrate Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched.
2026-06-10T07:47:12.199926+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:32.873625+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: sulfurisphaera_medium_ph_2_5, sulfurisphaera_medium_ph_5_0
2026-08-06T03:04:32.873666+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 93c5db9fdcc006bd07f0bc78b32dbf5e101d4f6cdadbc37ce74d3e57e4e2fceb