Overview
- External ontology
- TOGO:M1661 — Haroarcula Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1661 Original source: NBRC - NBRC_M865 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=865
Composition
flowchart LR
CultureMech_008219["haroarcula_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008219 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008219 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_008219 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_KCl["KCl"]
CultureMech_008219 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008219 --> ing4_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing4_MnCl2_4H2O -.-> CHEBI_86368
ing5_Sodium_glutamate["Sodium glutamate"]
CultureMech_008219 --> ing5_Sodium_glutamate
CHEBI_64220["CHEBI:64220"]
ing5_Sodium_glutamate -.-> CHEBI_64220
ing6_Trisodium_citrate["Trisodium citrate"]
CultureMech_008219 --> ing6_Trisodium_citrate
CHEBI_53258["CHEBI:53258"]
ing6_Trisodium_citrate -.-> CHEBI_53258
ing7_FeCl2_4H2O["FeCl2·4H2O"]
CultureMech_008219 --> ing7_FeCl2_4H2O
CHEBI_86249["CHEBI:86249"]
ing7_FeCl2_4H2O -.-> CHEBI_86249
ing8_Agar__if_needed_["Agar (if needed)"]
CultureMech_008219 --> ing8_Agar__if_needed_
ing9_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_008219 --> ing9_Bacto_Yeast_Extract__Difco_
ing10_Casamino_Acids__Difco_["Casamino Acids (Difco)"]
CultureMech_008219 --> ing10_Casamino_Acids__Difco_
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
20 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
200 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 4 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 5 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.26 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 6 |
Sodium glutamate
C5H8NO4.Na 169.112 g/mol
|
1 G_PER_L |
CHEBI:64220
monosodium glutamate
|
Role: Nitrogen source; Properties: Defined component, Organic compound, Simple component |
| 7 |
Trisodium citrate
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
Role: Buffer; Properties: Defined component, Organic compound, Simple component |
| 8 |
FeCl2·4H2O
Cl2Fe.4H2O 198.811 g/mol
|
36 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 9 | Agar (if needed) | 20 G_PER_L | Role: Solidifying component | |
| 10 | Bacto Yeast Extract (Difco) | 5 G_PER_L | Properties: Undefined component | |
| 11 | Casamino Acids (Difco) | 5 G_PER_L | Properties: Undefined component |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- haroarcula_medium (CultureMech:008219)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.087862Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1661 |
| 2026-01-28T19:48:25.962570Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:54.616456Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008219 |
| 2026-03-17T06:30:20.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:13.219814+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Haroarcula Medium" to "haroarcula_medium" for consistent matching |
| 2026-06-05T07:41:08.308685+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:48.664685+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-08-06T03:05:37.410463+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 32473d2954d573ed9e26d102b7df396b99fa19714db44762a2bef98ac5f9764d |