Overview
- External ontology
- TOGO:M1756 — KO2(NH4)
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1756 Original source: NBRC - NBRC_M968 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=968
Composition
flowchart LR
CultureMech_008320["ko2_nh4"]
ing0_Distilled_water["Distilled water"]
CultureMech_008320 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008320 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_KH2PO4["KH2PO4"]
CultureMech_008320 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008320 --> ing3_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing3_FeSO4_7H2O -.-> CHEBI_75836
ing4__NH4_2SO4["(NH4)2SO4"]
CultureMech_008320 --> ing4__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing4__NH4_2SO4 -.-> CHEBI_62946
ing5_CaCl2_6H2O["CaCl2·6H2O"]
CultureMech_008320 --> ing5_CaCl2_6H2O
CHEBI_91243["CHEBI:91243"]
ing5_CaCl2_6H2O -.-> CHEBI_91243
ing6_Agar__if_needed_["Agar (if needed)"]
CultureMech_008320 --> ing6_Agar__if_needed_
ing7_EDTA_2Na["EDTA-2Na"]
CultureMech_008320 --> ing7_EDTA_2Na
CHEBI_64734["CHEBI:64734"]
ing7_EDTA_2Na -.-> CHEBI_64734
ing8_H3BO3["H3BO3"]
CultureMech_008320 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008320 --> ing9_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing9_MnCl2_4H2O -.-> CHEBI_86368
ing10_ZnSO4_7H2O["ZnSO4·7H2O"]
CultureMech_008320 --> ing10_ZnSO4_7H2O
CHEBI_32312["CHEBI:32312"]
ing10_ZnSO4_7H2O -.-> CHEBI_32312
ing11_CuSO4_5H2O["CuSO4·5H2O"]
CultureMech_008320 --> ing11_CuSO4_5H2O
CHEBI_31440["CHEBI:31440"]
ing11_CuSO4_5H2O -.-> CHEBI_31440
ing12_MoO3["MoO3"]
CultureMech_008320 --> ing12_MoO3
CHEBI_30627["CHEBI:30627"]
ing12_MoO3 -.-> CHEBI_30627
Ingredients (13)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
2.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Simple component, Defined component [Merged 2 duplicates: 1.0, 1.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.27 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 4 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.02 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 5 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.32 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Defined component, Inorganic compound, Simple component |
| 6 |
CaCl2·6H2O
Ca.2Cl.6H2O 219.074 g/mol
|
0.11 G_PER_L |
CHEBI:91243
calcium chloride hexahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 7 | Agar (if needed) | 20 G_PER_L | Role: Solidifying component | |
| 8 |
EDTA-2Na
C10H14N2O8.2Na 336.208 g/mol
|
0.03 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
2.86 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
1.81 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 11 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.22 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
| 12 |
CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.08 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 13 |
MoO3
MoO3 143.937 g/mol
|
15 G_PER_L |
CHEBI:30627
molybdenum trioxide
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Simple component |
Solutions
N1 Metals solution*
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- ko2_nh4 (CultureMech:008320)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.283663Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1756 |
| 2026-01-28T19:48:09.016900Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:14.502242+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 1 solution(s) from ingredients |
| 2026-03-13T03:08:41.585867Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:55.651192Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008320 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name |
| 2026-03-17T06:29:43.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:14.190503+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "KO2(NH4)" to "ko2_nh4" for consistent matching |
| 2026-05-16T09:03:38.529642+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:10.455021+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:50.120470+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:47:00.389105+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:39.672282+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0b43facd6e2e73c64d3c686ae24925a44d9c3d6cc7ccce93eb5fae93d6f6ccb5 |