artificial_seawater_medium_for_strain_js_srb250lac (CultureMech:008354)

Overview

External ontology

TOGO:M1787 — Artificial seawater medium for strain JS_SRB250Lac

Applications

Microbial cultivation

High-metal flag

True

Notes

Source: https://togomedium.org/medium/M1787 Original source: NBRC - NBRC_M1005 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1005

Composition

flowchart LR
    CultureMech_008354["artificial_seawater_medium_for_strain_js_srb250lac"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008354 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_NaCl["NaCl"]
    CultureMech_008354 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_CaCl2_2H2O["CaCl2·2H2O"]
    CultureMech_008354 --> ing2_CaCl2_2H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_2H2O -.-> CHEBI_86158
    ing3_KH2PO4["KH2PO4"]
    CultureMech_008354 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_NH4Cl["NH4Cl"]
    CultureMech_008354 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_Resazurin["Resazurin"]
    CultureMech_008354 --> ing5_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing5_Resazurin -.-> CHEBI_8806
    ing6_MgCl2_6H2O["MgCl2·6H2O"]
    CultureMech_008354 --> ing6_MgCl2_6H2O
    CHEBI_86345["CHEBI:86345"]
    ing6_MgCl2_6H2O -.-> CHEBI_86345
    ing7_KCl["KCl"]
    CultureMech_008354 --> ing7_KCl
    CHEBI_32588["CHEBI:32588"]
    ing7_KCl -.-> CHEBI_32588
    ing8_NaHCO3["NaHCO3"]
    CultureMech_008354 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_Na2SO4["Na2SO4"]
    CultureMech_008354 --> ing9_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing9_Na2SO4 -.-> CHEBI_32149
    ing10_Sodium_lactate["Sodium lactate"]
    CultureMech_008354 --> ing10_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing10_Sodium_lactate -.-> CHEBI_75228
    ing11_KBr["KBr"]
    CultureMech_008354 --> ing11_KBr
    CHEBI_32030["CHEBI:32030"]
    ing11_KBr -.-> CHEBI_32030
    ing12_Carbon_dioxide_gas["Carbon dioxide gas"]
    CultureMech_008354 --> ing12_Carbon_dioxide_gas
    CHEBI_16526["CHEBI:16526"]
    ing12_Carbon_dioxide_gas -.-> CHEBI_16526
    ing13_Nitrogen_gas["Nitrogen gas"]
    CultureMech_008354 --> ing13_Nitrogen_gas
    CHEBI_17997["CHEBI:17997"]
    ing13_Nitrogen_gas -.-> CHEBI_17997
    ing14_Na2MoO4_2H2O["Na2MoO4·2H2O"]
    CultureMech_008354 --> ing14_Na2MoO4_2H2O
    CHEBI_75213["CHEBI:75213"]
    ing14_Na2MoO4_2H2O -.-> CHEBI_75213
    ing15_H3BO3["H3BO3"]
    CultureMech_008354 --> ing15_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing15_H3BO3 -.-> CHEBI_33118
    ing16_MnCl2_4H2O["MnCl2·4H2O"]
    CultureMech_008354 --> ing16_MnCl2_4H2O
    CHEBI_86368["CHEBI:86368"]
    ing16_MnCl2_4H2O -.-> CHEBI_86368
    ing17_CoCl2_6H2O["CoCl2·6H2O"]
    CultureMech_008354 --> ing17_CoCl2_6H2O
    CHEBI_35696["CHEBI:35696"]
    ing17_CoCl2_6H2O -.-> CHEBI_35696
    ing18_NiCl2_6H2O["NiCl2·6H2O"]
    CultureMech_008354 --> ing18_NiCl2_6H2O
    CHEBI_34887["CHEBI:34887"]
    ing18_NiCl2_6H2O -.-> CHEBI_34887
    ing19_CuCl2_2H2O["CuCl2·2H2O"]
    CultureMech_008354 --> ing19_CuCl2_2H2O
    CHEBI_86318["CHEBI:86318"]
    ing19_CuCl2_2H2O -.-> CHEBI_86318
    ing20_ZnCl2["ZnCl2"]
    CultureMech_008354 --> ing20_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing20_ZnCl2 -.-> CHEBI_49976
    ing21_HCl__25___7_7_M_["HCl (25%; 7.7 M)"]
    CultureMech_008354 --> ing21_HCl__25___7_7_M_
    ing22_FeCl2_4H2O["FeCl2·4H2O"]
    CultureMech_008354 --> ing22_FeCl2_4H2O
    CHEBI_86249["CHEBI:86249"]
    ing22_FeCl2_4H2O -.-> CHEBI_86249
    ing23_Na2SeO3_5H2O["Na2SeO3·5H2O"]
    CultureMech_008354 --> ing23_Na2SeO3_5H2O
    CHEBI_131361["CHEBI:131361"]
    ing23_Na2SeO3_5H2O -.-> CHEBI_131361
    ing24_Na2WO4_2H2O["Na2WO4·2H2O"]
    CultureMech_008354 --> ing24_Na2WO4_2H2O
    CHEBI_63939["CHEBI:63939"]
    ing24_Na2WO4_2H2O -.-> CHEBI_63939
    ing25_NaOH["NaOH"]
    CultureMech_008354 --> ing25_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing25_NaOH -.-> CHEBI_32145
    ing26_Biotin["Biotin"]
    CultureMech_008354 --> ing26_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing26_Biotin -.-> CHEBI_15956
    ing27_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_008354 --> ing27_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing27_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing28_Thiamine_HCl["Thiamine-HCl"]
    CultureMech_008354 --> ing28_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing28_Thiamine_HCl -.-> CHEBI_49105
    ing29_Ca_pantothenate["Ca-pantothenate"]
    CultureMech_008354 --> ing29_Ca_pantothenate
    CHEBI_29032["CHEBI:29032"]
    ing29_Ca_pantothenate -.-> CHEBI_29032
    CultureMech_008354 --> more["...9 more"]

Ingredients (39)

#	Ingredient	Concentration	Ontology	Notes
1	Distilled water H2O 18.015 g/mol	1093.0 G_PER_L	CHEBI:15377 water	Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 5 duplicates: 1.0, 990.0, 1.0, 1.0, 100.0]
2	NaCl Cl.Na 58.443 g/mol	26 G_PER_L	CHEBI:26710 sodium chloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
3	CaCl2·2H2O Ca.2Cl.2H2O 147.014 g/mol	1.4 G_PER_L	CHEBI:86158 calcium chloride dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
4	KH2PO4 H2O4P.K 136.084 g/mol	0.2 G_PER_L	CHEBI:63036 potassium dihydrogen phosphate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
5	NH4Cl Cl.H4N 53.492 g/mol	0.25 G_PER_L	CHEBI:31206 ammonium chloride	Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component
6	Resazurin C12H7NO4 229.191 g/mol	0.5 G_PER_L	CHEBI:8806 Resazurin	Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component
7	MgCl2·6H2O 2Cl.6H2O.Mg 203.301 g/mol	11.2 G_PER_L	CHEBI:86345 magnesium dichloride hexahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
8	KCl Cl.K 74.551 g/mol	0.7 G_PER_L	CHEBI:32588 potassium chloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
9	NaHCO3 CHO3.Na 84.006 g/mol	2.5 G_PER_L	CHEBI:32139 sodium hydrogencarbonate	Role: Buffer; Properties: Inorganic compound, Defined component, Simple component
10	Na2SO4 2Na.O4S 142.043 g/mol	4 G_PER_L	CHEBI:32149 sodium sulfate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
11	Sodium lactate C3H5O3.Na 112.06 g/mol	2.3 G_PER_L	CHEBI:75228 sodium lactate	Role: Carbon source; Properties: Organic compound, Defined component, Simple component
12	KBr Br.K 119.002 g/mol	0.1 G_PER_L	CHEBI:32030 potassium bromide	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
13	Carbon dioxide gas CO2 44.009 g/mol	variable VARIABLE	CHEBI:16526 carbon dioxide	Properties: Gas
14	Nitrogen gas N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Properties: ガス
15	Na2MoO4·2H2O 2H2O.MoO4.2Na 241.946 g/mol	36 G_PER_L	CHEBI:75213 sodium molybdate dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
16	H3BO3 H3BO3 61.833 g/mol	6 G_PER_L	CHEBI:33118 boric acid	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
17	MnCl2·4H2O Cl2Mn.4H2O 197.904 g/mol	100 G_PER_L	CHEBI:86368 manganese(II) chloride tetrahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
18	CoCl2·6H2O 2Cl.Co 129.839 g/mol	190 G_PER_L	CHEBI:35696 cobalt dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
19	NiCl2·6H2O Cl2Ni 129.599 g/mol	24 G_PER_L	CHEBI:34887 nickel dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
20	CuCl2·2H2O Cl2Cu.2H2O 170.482 g/mol	2 G_PER_L	CHEBI:86318 copper(II) chloride dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
21	ZnCl2 2Cl.Zn 136.296 g/mol	7 G_PER_L	CHEBI:49976 zinc dichloride	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
22	HCl (25%; 7.7 M)	10 G_PER_L		Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component
23	FeCl2·4H2O Cl2Fe.4H2O 198.811 g/mol	1.5 G_PER_L	CHEBI:86249 iron dichloride tetrahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
24	Na2SeO3·5H2O 5H2O.2Na.O3Se 263.012 g/mol	3 G_PER_L	CHEBI:131361 disodium selenite pentahydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
25	Na2WO4·2H2O 2H2O.2Na.O4W 329.846 g/mol	4 G_PER_L	CHEBI:63939 sodium tungstate dihydrate	Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component
26	NaOH HO.Na 39.997 g/mol	0.5 G_PER_L	CHEBI:32145 sodium hydroxide	Properties: Defined component, Inorganic compound, Simple component
27	Biotin C10H16N2O3S 244.316 g/mol	2 G_PER_L	CHEBI:15956 biotin	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
28	p-Aminobenzoic acid C7H7NO2 137.138 g/mol	5 G_PER_L	CHEBI:30753 4-aminobenzoic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
29	Thiamine-HCl C12H17N4OS.Cl.HCl 337.276 g/mol	5 G_PER_L	CHEBI:49105 thiamine hydrochloride	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
30	Ca-pantothenate C9H16NO5 218.229 g/mol	5 G_PER_L	CHEBI:29032 (R)-pantothenate	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
31	Pyridoxine-HCl C8H11NO3.HCl 205.641 g/mol	10 G_PER_L	CHEBI:30961 pyridoxine hydrochloride	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
32	Folic acid C19H19N7O6 441.404 g/mol	2 G_PER_L	CHEBI:27470 folic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
33	Vitamin B12	0.1 G_PER_L	CHEBI:176843 vitamin B12	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
34	Riboflavin C17H20N4O6 376.369 g/mol	5 G_PER_L	CHEBI:17015 riboflavin	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
35	Nicotinic acid C6H5NO2 123.111 g/mol	5 G_PER_L	CHEBI:15940 nicotinic acid	Role: Vitamin; Properties: Organic compound, Defined component, Simple component
36	Lipoic acid C8H14O2S2 206.332 g/mol	5 G_PER_L	CHEBI:16494 lipoic acid	Role: Growth factor; Properties: Organic compound, Defined component, Simple component
37	Na2S·9H2O 9H2O.2Na.S 240.182 g/mol	1.5 G_PER_L	CHEBI:76209 sodium sulfide nonahydrate	Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component
38	H2SO4 H2O4S 98.079 g/mol	variable VARIABLE	CHEBI:26836 sulfuric acid	Role: Mineral source; Properties: Defined component, Inorganic compound, Solution
39	N2 N2 28.014 g/mol	variable VARIABLE	CHEBI:17997 dinitrogen	Properties: ガス

Solutions

Trace element solution SL-10*

Dose: 1 G_PER_L

See mediadive_4178_Trace_element_solution_SL-10.yaml for composition

Selenite-tungstate solution**

Dose: 1 G_PER_L

See mediadive_5543_Selenite-tungstate_solution.yaml for composition

Vitamin solution***

Dose: 1 G_PER_L

See mediadive_6241_Vitamin_solution.yaml for composition

Sulfide solution****

Dose: 10 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

artificial_seawater_medium_for_strain_js_srb250lac (CultureMech:008354)

Timestamp	Curator	Action	Notes
2026-01-27T06:35:58.355865Z	togo-import	Imported from TOGO Medium	Source: NBRC, ID: M1787
2026-01-28T19:48:12.631337Z	chebi-enrichment	Added CHEBI ontology terms to ingredients	Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-02-03T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: N2
2026-03-09T08:04:20.861583+00:00	solution-migrator-v1.0	Migrated solutions from ingredients to solutions array	Moved 4 solution(s) from ingredients
2026-03-13T03:08:48.888945Z	data-quality-cleanup-v1.0	Data quality fixes	Merged 4 duplicate ingredient(s)
2026-03-13T06:19:56.004000Z	culturemech-id-assigner-v1.0	Assigned CultureMech ID	Assigned stable identifier: CultureMech:008354
2026-03-14T00:00:00+00:00	schema-defaulter-v1.0	Applied schema defaults and normalizations	Added default solution name; Added default solution name; Added default solution name; Added default solution name
2026-03-17T06:29:52.000000Z	mediaingredientmech-enrichment-v1.0	Added MediaIngredientMech links	Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:14.559660+00:00	normalize_media_names_to_snake_case.py	NAME_NORMALIZED	Normalized name from "Artificial seawater medium for strain JS_SRB250Lac" to "artificial_seawater_medium_for_strain_js_srb250lac" for consistent matching
2026-05-16T09:03:24.948906+00:00	migrate_legacy_fields.py	MIGRATED_LEGACY_FIELDS	curation_history.date->timestamp=2
2026-06-05T07:40:49.781903+00:00	mim-legacy-to-chebi-migration-v1.0	Refreshed legacy MediaIngredientMech links to CHEBI keying	Replaced 33 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:35.866471+00:00	chebi-regrounding-v1.0	Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24)	Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md.
2026-06-10T07:46:53.549226+00:00	primary-term-label-canonicalization-v1.0	Canonicalized primary term label to OBO canonical (OAK)	5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:24.060358+00:00	merge_recipes.py	MERGED_RECIPES	merged 1 source recipe(s) on fingerprint 4ebd55b82af9bbc86af529838a0183180b225b6e55e5564fe31441f5195b6128