Overview
- External ontology
- TOGO:M1789 — Medium for Ectothiorhodospira
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M1789 Original source: NBRC - NBRC_M1012 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1012
Composition
flowchart LR
CultureMech_008356["medium_for_ectothiorhodospira"]
ing0_Distilled_water["Distilled water"]
CultureMech_008356 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_008356 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008356 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_008356 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_008356 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_Resazurin["Resazurin"]
CultureMech_008356 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_Na2S_9H2O["Na2S·9H2O"]
CultureMech_008356 --> ing6_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing6_Na2S_9H2O -.-> CHEBI_76209
ing7_KCl["KCl"]
CultureMech_008356 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_NaHCO3["NaHCO3"]
CultureMech_008356 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_MgSO4["MgSO4"]
CultureMech_008356 --> ing9_MgSO4
CHEBI_32599["CHEBI:32599"]
ing9_MgSO4 -.-> CHEBI_32599
ing10_HCl["HCl"]
CultureMech_008356 --> ing10_HCl
CHEBI_17883["CHEBI:17883"]
ing10_HCl -.-> CHEBI_17883
ing11_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_008356 --> ing11_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing11_Carbon_dioxide_gas -.-> CHEBI_16526
ing12_Nitrogen_gas["Nitrogen gas"]
CultureMech_008356 --> ing12_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing12_Nitrogen_gas -.-> CHEBI_17997
ing13_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008356 --> ing13_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_2H2O -.-> CHEBI_75213
ing14_H3BO3["H3BO3"]
CultureMech_008356 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008356 --> ing15_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing15_FeSO4_7H2O -.-> CHEBI_75836
ing16_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008356 --> ing16_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing16_CoCl2_6H2O -.-> CHEBI_35696
ing17_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_008356 --> ing17_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing17_NiCl2_6H2O -.-> CHEBI_34887
ing18_CuCl2_2H2O["CuCl2·2H2O"]
CultureMech_008356 --> ing18_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing18_CuCl2_2H2O -.-> CHEBI_86318
ing19_ZnCl2["ZnCl2"]
CultureMech_008356 --> ing19_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing19_ZnCl2 -.-> CHEBI_49976
ing20_MnCl2_2H2O["MnCl2·2H2O"]
CultureMech_008356 --> ing20_MnCl2_2H2O
CHEBI_131395["CHEBI:131395"]
ing20_MnCl2_2H2O -.-> CHEBI_131395
ing21_Na2_EDTA["Na2-EDTA"]
CultureMech_008356 --> ing21_Na2_EDTA
CHEBI_64734["CHEBI:64734"]
ing21_Na2_EDTA -.-> CHEBI_64734
ing22_H2SO4["H2SO4"]
CultureMech_008356 --> ing22_H2SO4
CHEBI_26836["CHEBI:26836"]
ing22_H2SO4 -.-> CHEBI_26836
ing23_N2["N2"]
CultureMech_008356 --> ing23_N2
ing23_N2 -.-> CHEBI_17997
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1099.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 998.0, 1.0, 100.0] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
30 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.25 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.34 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.34 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.5 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 7 |
Na2S·9H2O
9H2O.2Na.S 240.182 g/mol
|
1.9 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.4, 1.5] |
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.34 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
MgSO4
Mg.O4S 120.368 g/mol
|
0.5 G_PER_L |
CHEBI:32599
magnesium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
HCl
HCl 36.461 g/mol
|
variable VARIABLE |
CHEBI:17883
hydrogen chloride
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Solution |
| 12 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: Gas |
| 13 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 14 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
18 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
300 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 16 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
1.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 17 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
190 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 18 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
24 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
CuCl2·2H2O
Cl2Cu.2H2O 170.482 g/mol
|
2 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
42 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
MnCl2·2H2O
Cl2Mn.2H2O 161.874 g/mol
|
50 G_PER_L |
CHEBI:131395
manganese(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
|
3 G_PER_L |
CHEBI:64734
EDTA disodium salt (anhydrous)
|
Properties: Defined component, Organic compound, Simple component |
| 23 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
| 24 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
Solutions
Vitamin B12 (0.002%) solution
Dose: 1 G_PER_L
Role: Vitamin; Properties: Organic compound, Defined component, Solution
Trace element solution SL-12B*
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1789_Medium_for_Ectothiorhodospira (CultureMech:008356)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.363398Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1789 |
| 2026-01-28T19:48:10.358638Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: HCl; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas; Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:16.907801+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:08:44.370229Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 3 duplicate ingredient(s) |
| 2026-03-13T06:19:56.034584Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008356 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:14.591003+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium for Ectothiorhodospira" to "medium_for_ectothiorhodospira" for consistent matching |
| 2026-05-16T09:03:11.233410+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:34.346445+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:48.195819+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:09.612352+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 3c2520300a4056718d35ca68fec5b483b5b9a341ac68a7205d49219dd2545ef6 |