Overview
- External ontology
- TOGO:M180 — Desulfurococcus Amylolyticus Medium
- Applications
-
- Microbial cultivation
- High-metal flag
- True
- Notes
- Source: https://togomedium.org/medium/M180 Original source: JCM - JCM_M187 Original URL: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=187
Composition
flowchart LR
CultureMech_008380["desulfurococcus_amylolyticus_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008380 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_NaCl["NaCl"]
CultureMech_008380 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_CaCl2_2H2O["CaCl2・2H2O"]
CultureMech_008380 --> ing2_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_2H2O -.-> CHEBI_86158
ing3_KH2PO4["KH2PO4"]
CultureMech_008380 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_008380 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_Resazurin["Resazurin"]
CultureMech_008380 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_MgCl2_6H2O["MgCl2・6H2O"]
CultureMech_008380 --> ing6_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_6H2O -.-> CHEBI_86345
ing7_Na2S_9H2O["Na2S・9H2O"]
CultureMech_008380 --> ing7_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing7_Na2S_9H2O -.-> CHEBI_76209
ing8_KCl["KCl"]
CultureMech_008380 --> ing8_KCl
CHEBI_32588["CHEBI:32588"]
ing8_KCl -.-> CHEBI_32588
ing9_NaHCO3["NaHCO3"]
CultureMech_008380 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_Sulfur__powder_["Sulfur (powder)"]
CultureMech_008380 --> ing10_Sulfur__powder_
CHEBI_33403["CHEBI:33403"]
ing10_Sulfur__powder_ -.-> CHEBI_33403
ing11_Yeast_extract__BD_Difco_["Yeast extract (BD-Difco)"]
CultureMech_008380 --> ing11_Yeast_extract__BD_Difco_
ing12_Bacto_peptone__BD_Difco_["Bacto peptone (BD-Difco)"]
CultureMech_008380 --> ing12_Bacto_peptone__BD_Difco_
ing13_H2SO4["H2SO4"]
CultureMech_008380 --> ing13_H2SO4
CHEBI_26836["CHEBI:26836"]
ing13_H2SO4 -.-> CHEBI_26836
ing14_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_008380 --> ing14_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing14_Carbon_dioxide_gas -.-> CHEBI_16526
ing15_Nitrogen_gas["Nitrogen gas"]
CultureMech_008380 --> ing15_Nitrogen_gas
CHEBI_17997["CHEBI:17997"]
ing15_Nitrogen_gas -.-> CHEBI_17997
ing16_25__HCl__7_7_M_["25% HCl (7.7 M)"]
CultureMech_008380 --> ing16_25__HCl__7_7_M_
ing17_FeCl2_4H2O["FeCl2・4H2O"]
CultureMech_008380 --> ing17_FeCl2_4H2O
CHEBI_86249["CHEBI:86249"]
ing17_FeCl2_4H2O -.-> CHEBI_86249
ing18_Na2MoO4_2H2O["Na2MoO4・2H2O"]
CultureMech_008380 --> ing18_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing18_Na2MoO4_2H2O -.-> CHEBI_75213
ing19_H3BO3["H3BO3"]
CultureMech_008380 --> ing19_H3BO3
CHEBI_33118["CHEBI:33118"]
ing19_H3BO3 -.-> CHEBI_33118
ing20_MnCl2_4H2O["MnCl2・4H2O"]
CultureMech_008380 --> ing20_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing20_MnCl2_4H2O -.-> CHEBI_86368
ing21_CoCl2_6H2O["CoCl2・6H2O"]
CultureMech_008380 --> ing21_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing21_CoCl2_6H2O -.-> CHEBI_35696
ing22_NiCl2_6H2O["NiCl2・6H2O"]
CultureMech_008380 --> ing22_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing22_NiCl2_6H2O -.-> CHEBI_34887
ing23_CuCl2_2H2O["CuCl2・2H2O"]
CultureMech_008380 --> ing23_CuCl2_2H2O
CHEBI_86318["CHEBI:86318"]
ing23_CuCl2_2H2O -.-> CHEBI_86318
ing24_ZnCl2["ZnCl2"]
CultureMech_008380 --> ing24_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing24_ZnCl2 -.-> CHEBI_49976
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1981.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 990.0, 990.0, 1.0] |
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
0.5 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
CaCl2・2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.44 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.33 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.33 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 7 |
MgCl2・6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.7 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Na2S・9H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.8 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 11 | Sulfur (powder) | 10 G_PER_L |
CHEBI:33403
elemental sulfur
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 12 | Yeast extract (BD-Difco) | 0.2 G_PER_L | Properties: Undefined component | |
| 13 | Bacto peptone (BD-Difco) | 5 G_PER_L | Properties: Undefined component | |
| 14 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
| 15 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 16 |
Nitrogen gas
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: ガス |
| 17 | 25% HCl (7.7 M) | 10 G_PER_L | Role: Solvating media; Properties: Defined component, Inorganic compound, Solution | |
| 18 |
FeCl2・4H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 19 |
Na2MoO4・2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
36 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 20 |
H3BO3
H3BO3 61.833 g/mol
|
6 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
MnCl2・4H2O
Cl2Mn.4H2O 197.904 g/mol
|
100 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
CoCl2・6H2O
2Cl.Co 129.839 g/mol
|
190 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
NiCl2・6H2O
Cl2Ni 129.599 g/mol
|
24 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
CuCl2・2H2O
Cl2Cu.2H2O 170.482 g/mol
|
2 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
70 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
Solutions
FeCl2 solution (see below)
Dose: 1 G_PER_L
Trace element solution (see below)
Dose: 1 G_PER_L
Trace vitamins (see Medium [M190])
Dose: 10 G_PER_L
Cross-reference to Medium M190
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M180_Desulfurococcus_Amylolyticus_Medium (CultureMech:008380)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:54.563621Z | togo-import | Imported from TOGO Medium | Source: JCM, ID: M180 |
| 2026-01-28T19:48:18.159981Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Nitrogen gas |
| 2026-03-09T08:04:30.099776+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:08:59.028421Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:56.301584Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008380 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:04.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:14.866020+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Desulfurococcus Amylolyticus Medium" to "desulfurococcus_amylolyticus_medium" for consistent matching |
| 2026-05-16T09:03:11.349864+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:34.462038+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:48.240221+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:25.754857+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0bb26d31ecae9ef3067e80066638c435b5fbd6a4886c290844bdbea745fa45ed |