Overview
- External ontology
- TOGO:M1817 — AS medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1817 Original source: NBRC - NBRC_M1049 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1049
Composition
flowchart LR
CultureMech_008388["as_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008388 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008388 --> ing1_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_7H2O -.-> CHEBI_31795
ing2_NaCl["NaCl"]
CultureMech_008388 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008388 --> ing3_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_2H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_008388 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_Resazurin["Resazurin"]
CultureMech_008388 --> ing5_Resazurin
CHEBI_8806["CHEBI:8806"]
ing5_Resazurin -.-> CHEBI_8806
ing6_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_008388 --> ing6_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_6H2O -.-> CHEBI_86345
ing7_Na2S_9H2O["Na2S·9H2O"]
CultureMech_008388 --> ing7_Na2S_9H2O
CHEBI_76209["CHEBI:76209"]
ing7_Na2S_9H2O -.-> CHEBI_76209
ing8_H3BO3["H3BO3"]
CultureMech_008388 --> ing8_H3BO3
CHEBI_33118["CHEBI:33118"]
ing8_H3BO3 -.-> CHEBI_33118
ing9_KCl["KCl"]
CultureMech_008388 --> ing9_KCl
CHEBI_32588["CHEBI:32588"]
ing9_KCl -.-> CHEBI_32588
ing10__NH4_2SO4["(NH4)2SO4"]
CultureMech_008388 --> ing10__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing10__NH4_2SO4 -.-> CHEBI_62946
ing11_Sulfur__powder_["Sulfur (powder)"]
CultureMech_008388 --> ing11_Sulfur__powder_
CHEBI_33403["CHEBI:33403"]
ing11_Sulfur__powder_ -.-> CHEBI_33403
ing12_NaBr["NaBr"]
CultureMech_008388 --> ing12_NaBr
CHEBI_63004["CHEBI:63004"]
ing12_NaBr -.-> CHEBI_63004
ing13_SrCl2_6H2O["SrCl2·6H2O"]
CultureMech_008388 --> ing13_SrCl2_6H2O
CHEBI_36385["CHEBI:36385"]
ing13_SrCl2_6H2O -.-> CHEBI_36385
ing14_Fe_III__ammonium_citrate["Fe(III) ammonium citrate"]
CultureMech_008388 --> ing14_Fe_III__ammonium_citrate
ing15_Bis_Tris_Propane["Bis Tris Propane"]
CultureMech_008388 --> ing15_Bis_Tris_Propane
ing16_Bacto_Yeast_Extract__Difco_["Bacto Yeast Extract (Difco)"]
CultureMech_008388 --> ing16_Bacto_Yeast_Extract__Difco_
ing17_H2SO4["H2SO4"]
CultureMech_008388 --> ing17_H2SO4
CHEBI_26836["CHEBI:26836"]
ing17_H2SO4 -.-> CHEBI_26836
ing18_N2["N2"]
CultureMech_008388 --> ing18_N2
CHEBI_17997["CHEBI:17997"]
ing18_N2 -.-> CHEBI_17997
ing19_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008388 --> ing19_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing19_Na2MoO4_2H2O -.-> CHEBI_75213
ing20_MnCl2_4H2O["MnCl2·4H2O"]
CultureMech_008388 --> ing20_MnCl2_4H2O
CHEBI_86368["CHEBI:86368"]
ing20_MnCl2_4H2O -.-> CHEBI_86368
ing21_CoCl2_6H2O["CoCl2·6H2O"]
CultureMech_008388 --> ing21_CoCl2_6H2O
CHEBI_35696["CHEBI:35696"]
ing21_CoCl2_6H2O -.-> CHEBI_35696
ing22_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_008388 --> ing22_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing22_NiCl2_6H2O -.-> CHEBI_34887
ing23_ZnCl2["ZnCl2"]
CultureMech_008388 --> ing23_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing23_ZnCl2 -.-> CHEBI_49976
ing24_FeCl3_6H2O["FeCl3·6H2O"]
CultureMech_008388 --> ing24_FeCl3_6H2O
CHEBI_86254["CHEBI:86254"]
ing24_FeCl3_6H2O -.-> CHEBI_86254
ing25_Nitrilotriacetic_acid__NTA_["Nitrilotriacetic acid (NTA)"]
CultureMech_008388 --> ing25_Nitrilotriacetic_acid__NTA_
CHEBI_44557["CHEBI:44557"]
ing25_Nitrilotriacetic_acid__NTA_ -.-> CHEBI_44557
ing26_Na2WO4["Na2WO4"]
CultureMech_008388 --> ing26_Na2WO4
CHEBI_63940["CHEBI:63940"]
ing26_Na2WO4 -.-> CHEBI_63940
ing27_AlK_SO4_2["AlK(SO4)2"]
CultureMech_008388 --> ing27_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing27_AlK_SO4_2 -.-> CHEBI_86463
ing28_Na2SeO3["Na2SeO3"]
CultureMech_008388 --> ing28_Na2SeO3
CHEBI_48843["CHEBI:48843"]
ing28_Na2SeO3 -.-> CHEBI_48843
ing29_NaOH["NaOH"]
CultureMech_008388 --> ing29_NaOH
CHEBI_32145["CHEBI:32145"]
ing29_NaOH -.-> CHEBI_32145
CultureMech_008388 --> more["...9 more"]
Ingredients (39)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
3.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 1.0, 1.0] |
| 2 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
6 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.318 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.3, 0.018] |
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.2 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 6 |
Resazurin
C12H7NO4 229.191 g/mol
|
1 G_PER_L |
CHEBI:8806
Resazurin
|
Role: pH dependent redox indicator; Properties: Organic compound, Defined component, Simple component |
| 7 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
3 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 8 |
Na2S·9H2O
9H2O.2Na.S 240.182 g/mol
|
0.3 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Role: Reducing agent; Properties: Inorganic compound, Defined component, Simple component |
| 9 |
H3BO3
H3BO3 61.833 g/mol
|
0.042 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.025, 0.017] |
| 10 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 11 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 12 | Sulfur (powder) | 10 G_PER_L |
CHEBI:33403
elemental sulfur
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 13 |
NaBr
Br.Na 102.894 g/mol
|
0.05 G_PER_L |
CHEBI:63004
sodium bromide
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 14 |
SrCl2·6H2O
Cl2Sr.6H2O 266.616 g/mol
|
0.02 G_PER_L |
CHEBI:36385
strontium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 15 | Fe(III) ammonium citrate | 0.01 G_PER_L | Role: Nitrogen source; Properties: Organic compound, Defined component, Simple component | |
| 16 | Bis Tris Propane | 2.25 G_PER_L | Role: Buffer; Properties: Organic compound, Defined component, Simple component | |
| 17 | Bacto Yeast Extract (Difco) | 2 G_PER_L | Properties: Undefined component | |
| 18 |
H2SO4
H2O4S 98.079 g/mol
|
variable VARIABLE |
CHEBI:26836
sulfuric acid
|
Role: Mineral source; Properties: Defined component, Inorganic compound, Solution |
| 19 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
| 20 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.009 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 21 |
MnCl2·4H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.09 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
CoCl2·6H2O
2Cl.Co 129.839 g/mol
|
0.15 G_PER_L |
CHEBI:35696
cobalt dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
0.018 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.09 G_PER_L |
CHEBI:49976
zinc dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
FeCl3·6H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.36 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 26 |
Nitrilotriacetic acid (NTA)
C6H9NO6 191.139 g/mol
|
4.1 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 27 |
Na2WO4
2Na.O4W 293.816 g/mol
|
1.6 G_PER_L |
CHEBI:63940
sodium tungstate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
5 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
Na2SeO3
2Na.O3Se 172.937 g/mol
|
1.6 G_PER_L |
CHEBI:48843
disodium selenite
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
Properties: Defined component, Inorganic compound, Solution |
| 31 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 32 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
1 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 33 |
Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
5 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 34 |
Ca-pantothenate
C9H16NO5 218.229 g/mol
|
5 G_PER_L |
CHEBI:29032
(R)-pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 35 |
Pyridoxine-HCl
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 36 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 37 | Vitamin B12 | 0.01 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 38 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 39 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
Solutions
Trace elements solution*
Dose: 10 G_PER_L
See mediadive_6129_Trace_elements_solution.yaml for composition
Vitamin solution**
Dose: 10 G_PER_L
See mediadive_6241_Vitamin_solution.yaml for composition
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- as_medium (CultureMech:008388)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:58.429275Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1817 |
| 2026-01-28T19:48:09.343217Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: H2SO4; Added default concentration for ingredient: N2; Added default concentration for ingredient: NaOH |
| 2026-03-09T08:04:15.130478+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T03:08:42.301434Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 4 duplicate ingredient(s) |
| 2026-03-13T06:19:56.374736Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008388 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:29:44.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:14.941756+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "AS medium" to "as_medium" for consistent matching |
| 2026-05-16T09:03:25.001769+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:49.849342+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:35.891502+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T06:57:36.864945+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:53.573046+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:24.136339+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d83812f19aa9f43fe61b6a400106d06d2bd055e128d56521b80db4a30e55a9b7 |