Overview
- External ontology
- TOGO:M1831 — Pae II Medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1831 Original source: NBRC - NBRC_M1064 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1064
Composition
flowchart LR
CultureMech_008404["pae_ii_medium"]
ing0_Distilled_water["Distilled water"]
CultureMech_008404 --> ing0_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing0_Distilled_water -.-> CHEBI_15377
ing1_Yeast_extract["Yeast extract"]
CultureMech_008404 --> ing1_Yeast_extract
ing2_NaCl["NaCl"]
CultureMech_008404 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_K2HPO4["K2HPO4"]
CultureMech_008404 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_MgSO4["MgSO4"]
CultureMech_008404 --> ing4_MgSO4
CHEBI_32599["CHEBI:32599"]
ing4_MgSO4 -.-> CHEBI_32599
ing5_Maltose["Maltose"]
CultureMech_008404 --> ing5_Maltose
CHEBI_18167["CHEBI:18167"]
ing5_Maltose -.-> CHEBI_18167
ing6_Agar__if_needed_["Agar (if needed)"]
CultureMech_008404 --> ing6_Agar__if_needed_
ing7_Tryptone["Tryptone"]
CultureMech_008404 --> ing7_Tryptone
ing8_Tris_HCl_buffer["Tris-HCl buffer"]
CultureMech_008404 --> ing8_Tris_HCl_buffer
CHEBI_9754["CHEBI:9754"]
ing8_Tris_HCl_buffer -.-> CHEBI_9754
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 2 | Yeast extract | 5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
10 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Simple component, Defined component, Inorganic compound |
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
1 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Defined component, Simple component, Inorganic compound |
| 5 |
MgSO4
Mg.O4S 120.368 g/mol
|
0.2 G_PER_L |
CHEBI:32599
magnesium sulfate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 6 |
Maltose
C12H22O11 342.297 g/mol
|
10 G_PER_L |
CHEBI:18167
alpha-maltose
|
Role: Carbon source; Properties: Organic compound, Simple component, Defined component |
| 7 | Agar (if needed) | 15 G_PER_L | Role: Solidifying component | |
| 8 | Tryptone | 5 G_PER_L | Properties: Undefined component | |
| 9 |
Tris-HCl buffer
C4H11NO3 121.136 g/mol
|
variable VARIABLE |
CHEBI:9754
tris
|
Role: Buffer; Properties: Complex component, Defined component, Solution |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- pae_ii_medium (CultureMech:008404)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.454683Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1831 |
| 2026-01-28T19:48:33.686868Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Tris-HCl buffer |
| 2026-03-13T06:19:56.489413Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008404 |
| 2026-03-17T06:30:36.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:15.058551+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Pae II Medium" to "pae_ii_medium" for consistent matching |
| 2026-05-16T09:04:28.343918+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:40.802458+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.850433+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:52.209952+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 78010edc11035cc83f2c6a2f74854596277ab08ca50b9124702e82e62a6f3dd8 |