Overview
- External ontology
- TOGO:M1836 — Medium for strains JA145, JA193, JA310, JA334, JA415, JA430, JA447 and JA643
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1836 Original source: NBRC - NBRC_M1068-2 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1068
Composition
flowchart LR
CultureMech_008409["medium_for_strains_ja145_ja193_ja310_ja334_ja415_ja430_ja447_and_ja643"]
ing0_Vitamin_B12__0_002___w_v__["Vitamin B12 (0.002% (w/v))"]
CultureMech_008409 --> ing0_Vitamin_B12__0_002___w_v__
ing1_Distilled_water["Distilled water"]
CultureMech_008409 --> ing1_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing1_Distilled_water -.-> CHEBI_15377
ing2_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008409 --> ing2_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_7H2O -.-> CHEBI_31795
ing3_Yeast_extract["Yeast extract"]
CultureMech_008409 --> ing3_Yeast_extract
ing4_NaCl["NaCl"]
CultureMech_008409 --> ing4_NaCl
CHEBI_26710["CHEBI:26710"]
ing4_NaCl -.-> CHEBI_26710
ing5_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008409 --> ing5_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_2H2O -.-> CHEBI_86158
ing6_KH2PO4["KH2PO4"]
CultureMech_008409 --> ing6_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing6_KH2PO4 -.-> CHEBI_63036
ing7_NH4Cl["NH4Cl"]
CultureMech_008409 --> ing7_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing7_NH4Cl -.-> CHEBI_31206
ing8_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_008409 --> ing8_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing8_Sodium_pyruvate -.-> CHEBI_50144
ing9_Na2S2O3["Na2S2O3"]
CultureMech_008409 --> ing9_Na2S2O3
CHEBI_32150["CHEBI:32150"]
ing9_Na2S2O3 -.-> CHEBI_32150
ing10_N2["N2"]
CultureMech_008409 --> ing10_N2
CHEBI_17997["CHEBI:17997"]
ing10_N2 -.-> CHEBI_17997
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Vitamin B12 (0.002% (w/v)) | 1 G_PER_L | Role: Vitamin; Properties: Defined component, Organic compound, Solution | |
| 2 |
Distilled water
H2O 18.015 g/mol
|
1 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component |
| 3 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Simple component, Inorganic compound, Defined component |
| 4 | Yeast extract | 0.5 G_PER_L | Role: Nutrient source; Properties: Undefined component | |
| 5 |
NaCl
Cl.Na 58.443 g/mol
|
0.4 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Defined component, Simple component, Inorganic compound |
| 6 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.05 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component |
| 7 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.5 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Simple component, Defined component |
| 8 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.6 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Simple component, Inorganic compound, Defined component |
| 9 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
3 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Role: Carbon source; Properties: Defined component, Organic compound, Simple component |
| 10 |
Na2S2O3
5H2O.2Na.O3S2 248.186 g/mol
|
variable VARIABLE |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Inorganic compound, Solution |
| 11 |
N2
N2 28.014 g/mol
|
variable VARIABLE |
CHEBI:17997
dinitrogen
|
Properties: Gas |
Solutions
Ferric citrate solution* (0.1%)
Dose: 5 G_PER_L
Role: Mineral source; Properties: Defined component, Solution, Organic compound
**Trace element solution SL7 (see Medium [M1835])
Dose: 1 G_PER_L
Cross-reference to Medium M1835
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1836_Medium_for_strains_JA145_JA193_JA310_JA334_JA415_JA430_JA447_and_JA643 (CultureMech:008409)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T06:35:58.471207Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1836 |
| 2026-01-28T19:48:16.487352Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Na2S2O3; Added default concentration for ingredient: N2 |
| 2026-03-09T08:04:27.241163+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 2 solution(s) from ingredients |
| 2026-03-13T06:19:56.567534Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008409 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name |
| 2026-03-17T06:30:00.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:15.137567+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "Medium for strains JA145, JA193, JA310, JA334, JA415, JA430, JA447 and JA643" to "medium_for_strains_ja145_ja193_ja310_ja334_ja415_ja430_ja447_and_ja643" for consistent matching |
| 2026-05-16T09:03:11.393983+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:34.505587+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 8 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T06:57:24.618109+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.259060+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:09.694789+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint a3326ef71fd18ead045c38f112c3a060cf636b02480f534f8340c6c2401fe881 |