Overview
- External ontology
- TOGO:M1846 — MMJHS medium
- Applications
-
- Microbial cultivation
- Notes
- Source: https://togomedium.org/medium/M1846 Original source: NBRC - NBRC_M1081 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1081
Composition
flowchart LR
CultureMech_008420["mmjhs_medium"]
ing0_NaHCO3["NaHCO3"]
CultureMech_008420 --> ing0_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing0_NaHCO3 -.-> CHEBI_32139
ing1_NaNO3["NaNO3"]
CultureMech_008420 --> ing1_NaNO3
CHEBI_63005["CHEBI:63005"]
ing1_NaNO3 -.-> CHEBI_63005
ing2_Sulfur__powder_["Sulfur (powder)"]
CultureMech_008420 --> ing2_Sulfur__powder_
CHEBI_33403["CHEBI:33403"]
ing2_Sulfur__powder_ -.-> CHEBI_33403
ing3_Na2S2O3_5H2O["Na2S2O3·5H2O"]
CultureMech_008420 --> ing3_Na2S2O3_5H2O
CHEBI_32150["CHEBI:32150"]
ing3_Na2S2O3_5H2O -.-> CHEBI_32150
ing4_Carbon_dioxide_gas["Carbon dioxide gas"]
CultureMech_008420 --> ing4_Carbon_dioxide_gas
CHEBI_16526["CHEBI:16526"]
ing4_Carbon_dioxide_gas -.-> CHEBI_16526
ing5_Hydrogen_gas["Hydrogen gas"]
CultureMech_008420 --> ing5_Hydrogen_gas
CHEBI_18276["CHEBI:18276"]
ing5_Hydrogen_gas -.-> CHEBI_18276
ing6_Distilled_water["Distilled water"]
CultureMech_008420 --> ing6_Distilled_water
CHEBI_15377["CHEBI:15377"]
ing6_Distilled_water -.-> CHEBI_15377
ing7_Biotin["Biotin"]
CultureMech_008420 --> ing7_Biotin
CHEBI_15956["CHEBI:15956"]
ing7_Biotin -.-> CHEBI_15956
ing8_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_008420 --> ing8_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing8_p_Aminobenzoic_acid -.-> CHEBI_30753
ing9_Thiamine_HCl["Thiamine-HCl"]
CultureMech_008420 --> ing9_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing9_Thiamine_HCl -.-> CHEBI_49105
ing10_Ca_pantothenate["Ca-pantothenate"]
CultureMech_008420 --> ing10_Ca_pantothenate
CHEBI_29032["CHEBI:29032"]
ing10_Ca_pantothenate -.-> CHEBI_29032
ing11_Pyridoxine_HCl["Pyridoxine-HCl"]
CultureMech_008420 --> ing11_Pyridoxine_HCl
CHEBI_30961["CHEBI:30961"]
ing11_Pyridoxine_HCl -.-> CHEBI_30961
ing12_Folic_acid["Folic acid"]
CultureMech_008420 --> ing12_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing12_Folic_acid -.-> CHEBI_27470
ing13_Vitamin_B12["Vitamin B12"]
CultureMech_008420 --> ing13_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing13_Vitamin_B12 -.-> CHEBI_176843
ing14_Riboflavin["Riboflavin"]
CultureMech_008420 --> ing14_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing14_Riboflavin -.-> CHEBI_17015
ing15_Nicotinic_acid["Nicotinic acid"]
CultureMech_008420 --> ing15_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing15_Nicotinic_acid -.-> CHEBI_15940
ing16_Lipoic_acid["Lipoic acid"]
CultureMech_008420 --> ing16_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing16_Lipoic_acid -.-> CHEBI_16494
ing17_MgSO4_7H2O["MgSO4·7H2O"]
CultureMech_008420 --> ing17_MgSO4_7H2O
CHEBI_31795["CHEBI:31795"]
ing17_MgSO4_7H2O -.-> CHEBI_31795
ing18_NaCl["NaCl"]
CultureMech_008420 --> ing18_NaCl
CHEBI_26710["CHEBI:26710"]
ing18_NaCl -.-> CHEBI_26710
ing19_CaCl2_2H2O["CaCl2·2H2O"]
CultureMech_008420 --> ing19_CaCl2_2H2O
CHEBI_86158["CHEBI:86158"]
ing19_CaCl2_2H2O -.-> CHEBI_86158
ing20_NH4Cl["NH4Cl"]
CultureMech_008420 --> ing20_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing20_NH4Cl -.-> CHEBI_31206
ing21_K2HPO4["K2HPO4"]
CultureMech_008420 --> ing21_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing21_K2HPO4 -.-> CHEBI_131527
ing22_MgCl2_6H2O["MgCl2·6H2O"]
CultureMech_008420 --> ing22_MgCl2_6H2O
CHEBI_86345["CHEBI:86345"]
ing22_MgCl2_6H2O -.-> CHEBI_86345
ing23_KCl["KCl"]
CultureMech_008420 --> ing23_KCl
CHEBI_32588["CHEBI:32588"]
ing23_KCl -.-> CHEBI_32588
ing24_NiCl2_6H2O["NiCl2·6H2O"]
CultureMech_008420 --> ing24_NiCl2_6H2O
CHEBI_34887["CHEBI:34887"]
ing24_NiCl2_6H2O -.-> CHEBI_34887
ing25_Na2SeO3_5H2O["Na2SeO3·5H2O"]
CultureMech_008420 --> ing25_Na2SeO3_5H2O
CHEBI_131361["CHEBI:131361"]
ing25_Na2SeO3_5H2O -.-> CHEBI_131361
ing26_Fe_NH4_2_SO4_2_6H2O["Fe(NH4)2(SO4)2·6H2O"]
CultureMech_008420 --> ing26_Fe_NH4_2_SO4_2_6H2O
CHEBI_76181["CHEBI:76181"]
ing26_Fe_NH4_2_SO4_2_6H2O -.-> CHEBI_76181
ing27_Na2MoO4_2H2O["Na2MoO4·2H2O"]
CultureMech_008420 --> ing27_Na2MoO4_2H2O
CHEBI_75213["CHEBI:75213"]
ing27_Na2MoO4_2H2O -.-> CHEBI_75213
ing28_H3BO3["H3BO3"]
CultureMech_008420 --> ing28_H3BO3
CHEBI_33118["CHEBI:33118"]
ing28_H3BO3 -.-> CHEBI_33118
ing29_FeSO4_7H2O["FeSO4·7H2O"]
CultureMech_008420 --> ing29_FeSO4_7H2O
CHEBI_75836["CHEBI:75836"]
ing29_FeSO4_7H2O -.-> CHEBI_75836
CultureMech_008420 --> more["...7 more"]
Ingredients (37)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaHCO3
CHO3.Na 84.006 g/mol
|
1 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 2 |
NaNO3
NO3.Na 84.994 g/mol
|
1 G_PER_L |
CHEBI:63005
sodium nitrate
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 3 | Sulfur (powder) | 3 G_PER_L |
CHEBI:33403
elemental sulfur
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 4 |
Na2S2O3·5H2O
5H2O.2Na.O3S2 248.186 g/mol
|
1 G_PER_L |
CHEBI:32150
sodium thiosulfate pentahydrate
|
Properties: Defined component, Inorganic compound, Simple component |
| 5 |
Carbon dioxide gas
CO2 44.009 g/mol
|
variable VARIABLE |
CHEBI:16526
carbon dioxide
|
Properties: ガス |
| 6 |
Hydrogen gas
H2 2.016 g/mol
|
variable VARIABLE |
CHEBI:18276
dihydrogen
|
Properties: ガス |
| 7 |
Distilled water
H2O 18.015 g/mol
|
992.0 G_PER_L |
CHEBI:15377
water
|
Role: Solvating media; Properties: Inorganic compound, Defined component, Simple component [Merged 3 duplicates: 1.0, 990.0, 1.0] |
| 8 |
Biotin
C10H16N2O3S 244.316 g/mol
|
2 G_PER_L |
CHEBI:15956
biotin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 9 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
5 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 10 |
Thiamine-HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
5 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 11 |
Ca-pantothenate
C9H16NO5 218.229 g/mol
|
5 G_PER_L |
CHEBI:29032
(R)-pantothenate
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 12 |
Pyridoxine-HCl
C8H11NO3.HCl 205.641 g/mol
|
10 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 13 |
Folic acid
C19H19N7O6 441.404 g/mol
|
2 G_PER_L |
CHEBI:27470
folic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 14 | Vitamin B12 | 0.1 G_PER_L |
CHEBI:176843
vitamin B12
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 15 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
5 G_PER_L |
CHEBI:17015
riboflavin
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 16 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
5 G_PER_L |
CHEBI:15940
nicotinic acid
|
Role: Vitamin; Properties: Organic compound, Defined component, Simple component |
| 17 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
5 G_PER_L |
CHEBI:16494
lipoic acid
|
Role: Growth factor; Properties: Organic compound, Defined component, Simple component |
| 18 |
MgSO4·7H2O
7H2O.Mg.O4S 246.473 g/mol
|
6.4 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 3.4, 3.0] |
| 19 |
NaCl
Cl.Na 58.443 g/mol
|
31.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 30.0, 1.0] |
| 20 |
CaCl2·2H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.24000000000000002 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.14, 0.1] |
| 21 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.25 G_PER_L |
CHEBI:31206
ammonium chloride
|
Role: Nitrogen source; Properties: Inorganic compound, Defined component, Simple component |
| 22 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.14 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
Role: Buffer; Properties: Inorganic compound, Defined component, Simple component |
| 23 |
MgCl2·6H2O
2Cl.6H2O.Mg 203.301 g/mol
|
4.18 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 24 |
KCl
Cl.K 74.551 g/mol
|
0.33 G_PER_L |
CHEBI:32588
potassium chloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 25 |
NiCl2·6H2O
Cl2Ni 129.599 g/mol
|
0.525 G_PER_L |
CHEBI:34887
nickel dichloride
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.5, 0.025] |
| 26 |
Na2SeO3·5H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.8 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component [Merged 2 duplicates: 0.5, 0.3] |
| 27 |
Fe(NH4)2(SO4)2·6H2O
Fe.6H2O.2H4N.2O4S 392.139 g/mol
|
0.01 G_PER_L |
CHEBI:76181
ferrous ammonium sulfate hexahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 28 |
Na2MoO4·2H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 29 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 30 |
FeSO4·7H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 31 |
ZnSO4·7H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 32 |
CuSO4·5H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 33 |
CoSO4·7H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 34 |
KAl(SO4)2·12H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 35 |
Nitrilotriacetic acid (NTA)
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
Role: Mineral source; Properties: Organic compound, Defined component, Simple component |
| 36 |
MnSO4·2H2O
2H2O.Mn.O4S 187.031 g/mol
|
0.5 G_PER_L |
CHEBI:86356
manganese(II) sulfate dihydrate
|
Role: Mineral source; Properties: Inorganic compound, Defined component, Simple component |
| 37 |
KOH
HO.K 56.105 g/mol
|
variable VARIABLE |
CHEBI:32035
potassium hydroxide
|
Role: Mineral source; Properties: Defined component, Solution |
Solutions
Balch's vitamin solution*
Dose: 10 G_PER_L
MJ synthetic seawater**
Dose: 990 G_PER_L
Balch's trace mineral solution***
Dose: 10 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- TOGO_M1846_MMJHS_medium (CultureMech:008420)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T06:35:58.495119Z | togo-import | Imported from TOGO Medium | Source: NBRC, ID: M1846 |
| 2026-01-28T19:48:38.446256Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-02-03T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: Carbon dioxide gas; Added default concentration for ingredient: Hydrogen gas; Added default concentration for ingredient: KOH |
| 2026-03-09T08:05:04.319115+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T03:09:36.279431Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 7 duplicate ingredient(s) |
| 2026-03-13T06:19:56.694723Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:008420 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:47.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:46:15.262403+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMJHS medium" to "mmjhs_medium" for consistent matching |
| 2026-05-16T09:03:11.457561+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=2 |
| 2026-06-05T07:40:34.573303+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:31.167752+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (shared-id regrounding audit, G21/G22/G24) | Part of the shared-id CHEBI regrounding audit (G21/G22/G24): one or more ingredient groundings on this record that had been collapsed onto a chemically non-specific or shared CHEBI id were re-grounded to the correct chemically-specific term, verified against OAK chebi.db rdfs:label. See reports/chebi_grounding_audit.md. |
| 2026-06-10T06:57:24.678953+00:00 | mgso4-heptahydrate-term-fix-v1.0 | Corrected MgSO4·7H2O primary term grounding to heptahydrate | Re-grounded 1 heptahydrate-labelled ingredient(s) from CHEBI:32599 (generic magnesium sulfate) to CHEBI:31795 (magnesium sulfate heptahydrate). Generic/hexahydrate labels untouched. |
| 2026-06-10T07:46:48.279547+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:09.771035+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 2514b7be3dfe98f6a67aab8d7e580908ea7941688e8a5473387b449f7a118ebd |