lb_medium_with_50_ppm_naphthalene

CultureMech:008421 bacterial COMPLEX SOLID_AGAR
Original name: LB Medium with 50 ppm Naphthalene

Overview

External ontology
TOGO:M1847 — LB Medium with 50 ppm Naphthalene
Applications
  • Microbial cultivation
Notes
Source: https://togomedium.org/medium/M1847 Original source: NBRC - NBRC_M1082 Original URL: https://www.nite.go.jp/nbrc/catalogue/NBRCMediumDetailServlet?NO=1082

Composition

flowchart LR
    CultureMech_008421["lb_medium_with_50_ppm_naphthalene"]
    ing0_Distilled_water["Distilled water"]
    CultureMech_008421 --> ing0_Distilled_water
    CHEBI_15377["CHEBI:15377"]
    ing0_Distilled_water -.-> CHEBI_15377
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_008421 --> ing1_Yeast_extract
    ing2_NaCl["NaCl"]
    CultureMech_008421 --> ing2_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing2_NaCl -.-> CHEBI_26710
    ing3_Agar__if_needed_["Agar (if needed)"]
    CultureMech_008421 --> ing3_Agar__if_needed_
    ing4_Peptone["Peptone"]
    CultureMech_008421 --> ing4_Peptone
    ing5_Ethanol["Ethanol"]
    CultureMech_008421 --> ing5_Ethanol
    CHEBI_16236["CHEBI:16236"]
    ing5_Ethanol -.-> CHEBI_16236
    ing6_Naphthalene["Naphthalene"]
    CultureMech_008421 --> ing6_Naphthalene
    CHEBI_16482["CHEBI:16482"]
    ing6_Naphthalene -.-> CHEBI_16482

Ingredients (7)

# Ingredient Concentration Ontology Notes
1 Distilled water
H2O 18.015 g/mol
1 G_PER_L CHEBI:15377
water
Role: Solvating media; Properties: Defined component, Inorganic compound, Simple component
2 Yeast extract 5 G_PER_L Role: Nutrient source; Properties: Undefined component
3 NaCl
Cl.Na 58.443 g/mol
5 G_PER_L CHEBI:26710
sodium chloride
Role: Mineral source; Properties: Inorganic compound, Simple component, Defined component
4 Agar (if needed) 15 G_PER_L Role: Solidifying component
5 Peptone 10 G_PER_L
6 Ethanol
C2H6O 46.069 g/mol
5 G_PER_L CHEBI:16236
ethanol
Role: Solvating media; Properties: Simple component, Organic compound, Defined component
7 Naphthalene
C10H8 128.174 g/mol
50 G_PER_L CHEBI:16482
naphthalene
Role: Carbon source; Properties: Organic compound, Simple component, Defined component

Solutions

Naphthalene solution (10,000 ppm)*

Dose: 5 G_PER_L

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-27T06:35:58.499940Z togo-import Imported from TOGO Medium Source: NBRC, ID: M1847
2026-01-28T19:48:18.624128Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-09T08:04:30.880222+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:56.705366Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:008421
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:05.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:46:15.273927+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "LB Medium with 50 ppm Naphthalene" to "lb_medium_with_50_ppm_naphthalene" for consistent matching
2026-05-16T09:03:38.873614+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:10.921937+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 4 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-08-06T03:05:40.056036+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 7972d1a54721f2e8d8b219cd6778036ceeab16235d3c3719fd2a7b2ee6d74db0